Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean

Exopolysaccharide (EPS) and lipopolysaccharide (LPS) from <i>Bradyrhizobium japonicum</i> are important for infection and nodulation of soybean (<i>Glycine max</i>), although their roles are not completely understood. To better understand this, we constructed mutants in <i...

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Autores principales: Quelas, Juan Ignacio, Mongiardini, Elías Javier, Casabuono, Adriana C., López García, Silvina Laura, Althabegoiti, María Julia, Covelli, Julieta Mariana, Pérez Giménez, Julieta, Couto, Alicia S., Lodeiro, Aníbal Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Ciencias Exactas
Biología
Química
exopolysaccharide
lipopolysaccharide
Bradyrhizobium japonicum
mutants
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/123573
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-123573
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Química
exopolysaccharide
lipopolysaccharide
Bradyrhizobium japonicum
mutants
spellingShingle Ciencias Exactas
Biología
Química
exopolysaccharide
lipopolysaccharide
Bradyrhizobium japonicum
mutants
Quelas, Juan Ignacio
Mongiardini, Elías Javier
Casabuono, Adriana C.
López García, Silvina Laura
Althabegoiti, María Julia
Covelli, Julieta Mariana
Pérez Giménez, Julieta
Couto, Alicia S.
Lodeiro, Aníbal Roberto
Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
topic_facet Ciencias Exactas
Biología
Química
exopolysaccharide
lipopolysaccharide
Bradyrhizobium japonicum
mutants
description Exopolysaccharide (EPS) and lipopolysaccharide (LPS) from <i>Bradyrhizobium japonicum</i> are important for infection and nodulation of soybean (<i>Glycine max</i>), although their roles are not completely understood. To better understand this, we constructed mutants in <i>B. japonicum</i> USDA 110 impaired in galactose or galacturonic acid incorporation into the EPS without affecting the LPS. The derivative LP 3010 had a deletion of <i>lspL-ugdH</i> and produced EPS without galacturonic acid whereas LP 3013, with an insertion in <i>exoB</i>, produced EPS without galactose. In addition, the strain LP 3017, with both mutations, had EPS devoid of both galactosides. The missing galactosides were not replaced by other sugars. The defects in EPS had different consequences. LP 3010 formed biofilms and nodulated but was defective in competitiveness for nodulation; and, inside nodules, the peribacteroid membranes tended to fuse, leading to the merging of symbiosomes. Meanwhile, LP 3013 and LP 3017 were unable to form biofilms and produced empty pseudonodules but <i>exoB</i> suppressor mutants were obtained when LP 3013 plant inoculation was supplemented with wild-type EPS. Similar phenotypes were observed with all these mutants in <i>G. soja</i>. Therefore, the lack of each galactoside in the EPS has a different functional effect on the <i>B. japonicum</i>-soybean symbiosis.
format Articulo
Articulo
author Quelas, Juan Ignacio
Mongiardini, Elías Javier
Casabuono, Adriana C.
López García, Silvina Laura
Althabegoiti, María Julia
Covelli, Julieta Mariana
Pérez Giménez, Julieta
Couto, Alicia S.
Lodeiro, Aníbal Roberto
author_facet Quelas, Juan Ignacio
Mongiardini, Elías Javier
Casabuono, Adriana C.
López García, Silvina Laura
Althabegoiti, María Julia
Covelli, Julieta Mariana
Pérez Giménez, Julieta
Couto, Alicia S.
Lodeiro, Aníbal Roberto
author_sort Quelas, Juan Ignacio
title Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
title_short Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
title_full Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
title_fullStr Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
title_full_unstemmed Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
title_sort lack of galactose or galacturonic acid in <i>bradyrhizobium japonicum</i> usda 110 exopolysaccharide leads to different symbiotic responses in soybean
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/123573
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