Structural Characterization of TssL from <i>Acinetobacter baumannii</i>: a Key Component of the Type VI Secretion System

The type VI secretion system (T6SS) is a complex molecular nanomachine used by Gram-negative bacteria to deliver diverse effectors into adjacent cells. A membrane complex (MC) anchors this transport system to the bacterial cell wall. One of the proteins forming the MC is TssL, a cytoplasmic protein...

Descripción completa

Detalles Bibliográficos
Autores principales: Ruiz, Federico M., Lopez, Juvenal, Ferrara, Carlos Gastón, Santillana, Elena, Espinosa Silva, Yanis Ricardo, Feldman, Mario F., Romero, Antonio A.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Exactas
Física
Química
Acinetobacter
secretion systems
structural biology
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/123516
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-123516
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Física
Química
Acinetobacter
secretion systems
structural biology
spellingShingle Ciencias Exactas
Física
Química
Acinetobacter
secretion systems
structural biology
Ruiz, Federico M.
Lopez, Juvenal
Ferrara, Carlos Gastón
Santillana, Elena
Espinosa Silva, Yanis Ricardo
Feldman, Mario F.
Romero, Antonio A.
Structural Characterization of TssL from <i>Acinetobacter baumannii</i>: a Key Component of the Type VI Secretion System
topic_facet Ciencias Exactas
Física
Química
Acinetobacter
secretion systems
structural biology
description The type VI secretion system (T6SS) is a complex molecular nanomachine used by Gram-negative bacteria to deliver diverse effectors into adjacent cells. A membrane complex (MC) anchors this transport system to the bacterial cell wall. One of the proteins forming the MC is TssL, a cytoplasmic protein bound to the inner membrane through a single transmembrane helix. Here, we report the structure of the cytoplasmic N-terminal region of TssL from <i>Acinetobacter baumannii</i>, a bacterium encoding in a single locus a secretion system that is a special case among other T6SSs. The protein structure, consisting of two antiparallel alpha-helical bundles connected by a short loop, reveals several interesting particularities compared with homologous proteins from other organisms. In addition, we demonstrate the structural significance of residues Asp98 and Glu99, which are strongly conserved among T6SS-encoding Gram-negative bacteria. Mutations in these two residues strongly impact protein dynamics, expression, and functionality. Our results improve our understanding of the T6SS of <i>A. baumannii</i>, which remains largely understudied compared with that of other pathogens. Several <i>Acinetobacter</i> species carry one functional type VI secretion system (T6SS). The T6SS is encoded in a single locus containing 16 conserved genes, most of which code for proteins essential to T6SS activity. One of these key components is TssL, a cytoplasmic protein bound to the inner membrane. Despite its importance and its particular characteristics, the structure of T6SS in <i>A. baumannii</i> remains understudied. Here, we present structural, <i>in silico</i>, and <i>in vivo</i> studies of TssL, highlighting the importance of two well-conserved residues and improving our understanding of this secretion system in this bacterium.
format Articulo
Articulo
author Ruiz, Federico M.
Lopez, Juvenal
Ferrara, Carlos Gastón
Santillana, Elena
Espinosa Silva, Yanis Ricardo
Feldman, Mario F.
Romero, Antonio A.
author_facet Ruiz, Federico M.
Lopez, Juvenal
Ferrara, Carlos Gastón
Santillana, Elena
Espinosa Silva, Yanis Ricardo
Feldman, Mario F.
Romero, Antonio A.
author_sort Ruiz, Federico M.
title Structural Characterization of TssL from <i>Acinetobacter baumannii</i>: a Key Component of the Type VI Secretion System
title_short Structural Characterization of TssL from <i>Acinetobacter baumannii</i>: a Key Component of the Type VI Secretion System
title_full Structural Characterization of TssL from <i>Acinetobacter baumannii</i>: a Key Component of the Type VI Secretion System
title_fullStr Structural Characterization of TssL from <i>Acinetobacter baumannii</i>: a Key Component of the Type VI Secretion System
title_full_unstemmed Structural Characterization of TssL from <i>Acinetobacter baumannii</i>: a Key Component of the Type VI Secretion System
title_sort structural characterization of tssl from <i>acinetobacter baumannii</i>: a key component of the type vi secretion system
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/123516
work_keys_str_mv AT ruizfedericom structuralcharacterizationoftsslfromiacinetobacterbaumanniiiakeycomponentofthetypevisecretionsystem
AT lopezjuvenal structuralcharacterizationoftsslfromiacinetobacterbaumanniiiakeycomponentofthetypevisecretionsystem
AT ferraracarlosgaston structuralcharacterizationoftsslfromiacinetobacterbaumanniiiakeycomponentofthetypevisecretionsystem
AT santillanaelena structuralcharacterizationoftsslfromiacinetobacterbaumanniiiakeycomponentofthetypevisecretionsystem
AT espinosasilvayanisricardo structuralcharacterizationoftsslfromiacinetobacterbaumanniiiakeycomponentofthetypevisecretionsystem
AT feldmanmariof structuralcharacterizationoftsslfromiacinetobacterbaumanniiiakeycomponentofthetypevisecretionsystem
AT romeroantonioa structuralcharacterizationoftsslfromiacinetobacterbaumanniiiakeycomponentofthetypevisecretionsystem
bdutipo_str Repositorios
_version_ 1764820449667055618

Ejemplares similares