Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife

In recent years, the human presence in Antarctica has increased and as a consequence, the possibility of microorganisms’ introduction. The aims of this work were to determine the presence of <i>Salmonella enterica</i> in Antarctic seabirds and sea mammals, to characterize the isolates id...

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Autores principales: Vigo, Germán Blas, Leotta, Gerardo Aníbal, Caffer, M. I., Salve, Angela, Binsztein, Norma, Pichel, Mariana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
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Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/123456
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id I19-R120-10915-123456
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Salmonella
Antarctica
PFGE
RAPD-PCR
spellingShingle Veterinaria
Salmonella
Antarctica
PFGE
RAPD-PCR
Vigo, Germán Blas
Leotta, Gerardo Aníbal
Caffer, M. I.
Salve, Angela
Binsztein, Norma
Pichel, Mariana
Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife
topic_facet Veterinaria
Salmonella
Antarctica
PFGE
RAPD-PCR
description In recent years, the human presence in Antarctica has increased and as a consequence, the possibility of microorganisms’ introduction. The aims of this work were to determine the presence of <i>Salmonella enterica</i> in Antarctic seabirds and sea mammals, to characterize the isolates identified, and to determine the genetic relation of Antarctic <i>S. enterica</i> isolates among them and compare with isolates of human, animal, and food sources recovered in Argentina. During the summer 2000 and 2002 in Potter Peninsula, and during the summer 2001 and 2003 in Hope Bay, a total of 1,739 fecal samples from Antarctic animals were collected and analyzed. In summer 2000, <i>S.</i> Newport and <i>S.</i> Enteritidis were isolated from 8.9% of southern giant petrels (<i>Macronectes giganteus</i>). In summer 2003, <i>S.</i> Enteritidis was isolated from 1.5% of Adelie penguins (<i>Pygoscelis adeliae</i>), from 5.5% of skuas (<i>Stercorarius</i> sp.), from 5.4% of kelp gulls (<i>Larus dominicanus</i>), and from 5.6% of Weddell seals (<i>Leptonychotes weddelli</i>). All the isolates belonging to the same serovar showed indistinguishable genomic profiles by Pulse-Field Gel Electrophoresis (PFGE) with <i>Xba</i>I and <i>Bln</i>I restriction enzymes and by Random Amplified Polymorphic DNA (RAPD-PCR). In addition, these Antarctic strains were different from <i>S. enterica</i> isolates from different sources identified in Argentina during the same or close time periods.
format Articulo
Articulo
author Vigo, Germán Blas
Leotta, Gerardo Aníbal
Caffer, M. I.
Salve, Angela
Binsztein, Norma
Pichel, Mariana
author_facet Vigo, Germán Blas
Leotta, Gerardo Aníbal
Caffer, M. I.
Salve, Angela
Binsztein, Norma
Pichel, Mariana
author_sort Vigo, Germán Blas
title Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife
title_short Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife
title_full Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife
title_fullStr Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife
title_full_unstemmed Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife
title_sort isolation and characterization of <i>salmonella enterica</i> from antarctic wildlife
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/123456
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