Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife
In recent years, the human presence in Antarctica has increased and as a consequence, the possibility of microorganisms’ introduction. The aims of this work were to determine the presence of <i>Salmonella enterica</i> in Antarctic seabirds and sea mammals, to characterize the isolates id...
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Formato: | Articulo |
Lenguaje: | Inglés |
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2010
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/123456 |
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I19-R120-10915-123456 |
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Universidad Nacional de La Plata |
institution_str |
I-19 |
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R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Veterinaria Salmonella Antarctica PFGE RAPD-PCR |
spellingShingle |
Veterinaria Salmonella Antarctica PFGE RAPD-PCR Vigo, Germán Blas Leotta, Gerardo Aníbal Caffer, M. I. Salve, Angela Binsztein, Norma Pichel, Mariana Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife |
topic_facet |
Veterinaria Salmonella Antarctica PFGE RAPD-PCR |
description |
In recent years, the human presence in Antarctica has increased and as a consequence, the possibility of microorganisms’ introduction. The aims of this work were to determine the presence of <i>Salmonella enterica</i> in Antarctic seabirds and sea mammals, to characterize the isolates identified, and to determine the genetic relation of Antarctic <i>S. enterica</i> isolates among them and compare with isolates of human, animal, and food sources recovered in Argentina. During the summer 2000 and 2002 in Potter Peninsula, and during the summer 2001 and 2003 in Hope Bay, a total of 1,739 fecal samples from Antarctic animals were collected and analyzed. In summer 2000, <i>S.</i> Newport and <i>S.</i> Enteritidis were isolated from 8.9% of southern giant petrels (<i>Macronectes giganteus</i>). In summer 2003, <i>S.</i> Enteritidis was isolated from 1.5% of Adelie penguins (<i>Pygoscelis adeliae</i>), from 5.5% of skuas (<i>Stercorarius</i> sp.), from 5.4% of kelp gulls (<i>Larus dominicanus</i>), and from 5.6% of Weddell seals (<i>Leptonychotes weddelli</i>). All the isolates belonging to the same serovar showed indistinguishable genomic profiles by Pulse-Field Gel Electrophoresis (PFGE) with <i>Xba</i>I and <i>Bln</i>I restriction enzymes and by Random Amplified Polymorphic DNA (RAPD-PCR). In addition, these Antarctic strains were different from <i>S. enterica</i> isolates from different sources identified in Argentina during the same or close time periods. |
format |
Articulo Articulo |
author |
Vigo, Germán Blas Leotta, Gerardo Aníbal Caffer, M. I. Salve, Angela Binsztein, Norma Pichel, Mariana |
author_facet |
Vigo, Germán Blas Leotta, Gerardo Aníbal Caffer, M. I. Salve, Angela Binsztein, Norma Pichel, Mariana |
author_sort |
Vigo, Germán Blas |
title |
Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife |
title_short |
Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife |
title_full |
Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife |
title_fullStr |
Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife |
title_full_unstemmed |
Isolation and characterization of <i>Salmonella enterica</i> from Antarctic wildlife |
title_sort |
isolation and characterization of <i>salmonella enterica</i> from antarctic wildlife |
publishDate |
2010 |
url |
http://sedici.unlp.edu.ar/handle/10915/123456 |
work_keys_str_mv |
AT vigogermanblas isolationandcharacterizationofisalmonellaentericaifromantarcticwildlife AT leottagerardoanibal isolationandcharacterizationofisalmonellaentericaifromantarcticwildlife AT caffermi isolationandcharacterizationofisalmonellaentericaifromantarcticwildlife AT salveangela isolationandcharacterizationofisalmonellaentericaifromantarcticwildlife AT binszteinnorma isolationandcharacterizationofisalmonellaentericaifromantarcticwildlife AT pichelmariana isolationandcharacterizationofisalmonellaentericaifromantarcticwildlife |
bdutipo_str |
Repositorios |
_version_ |
1764820449545420801 |