Immunodetection of phosphorylation sites gives new insights into the mechanisms underlying phospholamban phosphorylation in the intact heart
Phosphorylation site-specific antibodies, quantification of <sup>32</sup>P incorporation into phospholamban, and simultaneous measurements of mechanical activity were used in Langendorff-perfused rat hearts to provide further insights into the underlying mechanisms of phospholamban phosp...
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| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
1996
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/123153 |
| Aporte de: |
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I19-R120-10915-123153 |
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dspace |
| institution |
Universidad Nacional de La Plata |
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I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Medicina Biología Rats Phosphorylation Heart Phospholamban Isoproterenol |
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Medicina Biología Rats Phosphorylation Heart Phospholamban Isoproterenol Mundiña-Weilenmann, Cecilia Vittone, Leticia Beatriz Ortale, Manuel Chiappe de Cingolani, Gladys Ethel Mattiazzi, Alicia Ramona Immunodetection of phosphorylation sites gives new insights into the mechanisms underlying phospholamban phosphorylation in the intact heart |
| topic_facet |
Medicina Biología Rats Phosphorylation Heart Phospholamban Isoproterenol |
| description |
Phosphorylation site-specific antibodies, quantification of <sup>32</sup>P incorporation into phospholamban, and simultaneous measurements of mechanical activity were used in Langendorff-perfused rat hearts to provide further insights into the underlying mechanisms of phospholamban phosphorylation. Immunological detection of phospholamban phosphorylation sites showed that the isoproterenol concentration-dependent increase in phospholamban phosphorylation was due to increases in phosphorylation of both Ser<sup>16</sup> and Thr<sup>17</sup> residues. When isoproterenol concentration was increased at extremely low Ca²⁺ supply to the myocardium, phosphorylation of Thr<sup>17</sup> was virtually absent. Under these conditions, <sup>32</sup>P incorporation into phospholamban, due to Ser<sup>16</sup>, decreased by 50%. Changes in Ca²⁺ supply to the myocardium either at constant β-adrenergic stimulation or in the presence of okadaic acid, a phosphatase inhibitor, exclusively modified Thr<sup>17</sup> phosphorylation. Changes in phospholamban phosphorylation due to either Ser<sup>16</sup> and/or Thr<sup>17</sup> were paralleled by changes in myocardial relaxation. The results indicate that cAMP- (Ser<sup>16</sup>) and Ca²⁺-calmodulin (Thr<sup>17</sup>)-dependent pathways of phospholamban phosphorylation can occur independently of each other. However, in the absence of β-adrenergic stimulation, phosphorylation of Thr<sup>17</sup> could only be detected after simultaneous activation of Ca²⁺-calmodulin-dependent protein kinase and inactivation of phosphatase. It is suggested that under physiological conditions, this requisite is only filled by cAMP-dependent mechanisms. |
| format |
Articulo Articulo |
| author |
Mundiña-Weilenmann, Cecilia Vittone, Leticia Beatriz Ortale, Manuel Chiappe de Cingolani, Gladys Ethel Mattiazzi, Alicia Ramona |
| author_facet |
Mundiña-Weilenmann, Cecilia Vittone, Leticia Beatriz Ortale, Manuel Chiappe de Cingolani, Gladys Ethel Mattiazzi, Alicia Ramona |
| author_sort |
Mundiña-Weilenmann, Cecilia |
| title |
Immunodetection of phosphorylation sites gives new insights into the mechanisms underlying phospholamban phosphorylation in the intact heart |
| title_short |
Immunodetection of phosphorylation sites gives new insights into the mechanisms underlying phospholamban phosphorylation in the intact heart |
| title_full |
Immunodetection of phosphorylation sites gives new insights into the mechanisms underlying phospholamban phosphorylation in the intact heart |
| title_fullStr |
Immunodetection of phosphorylation sites gives new insights into the mechanisms underlying phospholamban phosphorylation in the intact heart |
| title_full_unstemmed |
Immunodetection of phosphorylation sites gives new insights into the mechanisms underlying phospholamban phosphorylation in the intact heart |
| title_sort |
immunodetection of phosphorylation sites gives new insights into the mechanisms underlying phospholamban phosphorylation in the intact heart |
| publishDate |
1996 |
| url |
http://sedici.unlp.edu.ar/handle/10915/123153 |
| work_keys_str_mv |
AT mundinaweilenmanncecilia immunodetectionofphosphorylationsitesgivesnewinsightsintothemechanismsunderlyingphospholambanphosphorylationintheintactheart AT vittoneleticiabeatriz immunodetectionofphosphorylationsitesgivesnewinsightsintothemechanismsunderlyingphospholambanphosphorylationintheintactheart AT ortalemanuel immunodetectionofphosphorylationsitesgivesnewinsightsintothemechanismsunderlyingphospholambanphosphorylationintheintactheart AT chiappedecingolanigladysethel immunodetectionofphosphorylationsitesgivesnewinsightsintothemechanismsunderlyingphospholambanphosphorylationintheintactheart AT mattiazzialiciaramona immunodetectionofphosphorylationsitesgivesnewinsightsintothemechanismsunderlyingphospholambanphosphorylationintheintactheart |
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Repositorios |
| _version_ |
1764820449830633472 |