Characterization of the overlapping promoters of nolB and nolW, two soybean cultivar specificity genes from Rhizobium fredii strain USDA257

The transcripts of nolW and nolB, two divergently oriented cultivar specificity genes of Rhizobium fredii strain USDA257, are known to be initiated 14 bp apart from promoters that face one another. We show here that expression of nolB is dependent both on induction with flavonoid signals and on the...

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Detalles Bibliográficos
Autores principales: Gu, Jun, Balatti, Pedro Alberto, Krishnan, Hari B., Pueppke, Steven G.
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 1997
Materias:
DNA
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/123050
Aporte de:
id I19-R120-10915-123050
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Promoter
Gene
Gene expression
Regulatory sequence
Rhizobiaceae
Rhizobium
Regulator gene
Genetics
DNA
Biology
spellingShingle Biología
Promoter
Gene
Gene expression
Regulatory sequence
Rhizobiaceae
Rhizobium
Regulator gene
Genetics
DNA
Biology
Gu, Jun
Balatti, Pedro Alberto
Krishnan, Hari B.
Pueppke, Steven G.
Characterization of the overlapping promoters of nolB and nolW, two soybean cultivar specificity genes from Rhizobium fredii strain USDA257
topic_facet Biología
Promoter
Gene
Gene expression
Regulatory sequence
Rhizobiaceae
Rhizobium
Regulator gene
Genetics
DNA
Biology
description The transcripts of nolW and nolB, two divergently oriented cultivar specificity genes of Rhizobium fredii strain USDA257, are known to be initiated 14 bp apart from promoters that face one another. We show here that expression of nolB is dependent both on induction with flavonoid signals and on the regulatory gene, nodD1. Expression of nolW is constitutive and independent of flavonoids and nodD1. Normal expression of nolB is retained with a promoter that extends only 61 bp upstream of the transcript start site, but it is lost if an additional 24 bp are removed. Substantial expression of nolW is retained with a promoter that contains only 34 bp of DNA upstream from the transcript initiation site. The dual control region for the two genes is thus only about 109 bp in length.
format Articulo
Comunicacion
author Gu, Jun
Balatti, Pedro Alberto
Krishnan, Hari B.
Pueppke, Steven G.
author_facet Gu, Jun
Balatti, Pedro Alberto
Krishnan, Hari B.
Pueppke, Steven G.
author_sort Gu, Jun
title Characterization of the overlapping promoters of nolB and nolW, two soybean cultivar specificity genes from Rhizobium fredii strain USDA257
title_short Characterization of the overlapping promoters of nolB and nolW, two soybean cultivar specificity genes from Rhizobium fredii strain USDA257
title_full Characterization of the overlapping promoters of nolB and nolW, two soybean cultivar specificity genes from Rhizobium fredii strain USDA257
title_fullStr Characterization of the overlapping promoters of nolB and nolW, two soybean cultivar specificity genes from Rhizobium fredii strain USDA257
title_full_unstemmed Characterization of the overlapping promoters of nolB and nolW, two soybean cultivar specificity genes from Rhizobium fredii strain USDA257
title_sort characterization of the overlapping promoters of nolb and nolw, two soybean cultivar specificity genes from rhizobium fredii strain usda257
publishDate 1997
url http://sedici.unlp.edu.ar/handle/10915/123050
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