Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)

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Cytogenetic studies in seawater or land planarians have been generally performed by means of the squash technique (Ball and De Vries 1983; Curini-Galletti et at., 1985, 1989a, b; Martens et at. 1989a, b; Oki et at. 1991; Puccinelli and Curum-Galletti 1987; Tamura et at. 1991). However, the quality o...

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Detalles Bibliográficos
Autores principales: Margaría, Cecilia Beatriz, Dulout, Fernando Noel
Formato: Articulo
Lenguaje:Inglés
Publicado: 1997
Materias:
Biología
Air drying technique
Planarians chromosomes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/122975
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-122975
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Air drying technique
Planarians chromosomes
spellingShingle Biología
Air drying technique
Planarians chromosomes
Margaría, Cecilia Beatriz
Dulout, Fernando Noel
Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
topic_facet Biología
Air drying technique
Planarians chromosomes
description Cytogenetic studies in seawater or land planarians have been generally performed by means of the squash technique (Ball and De Vries 1983; Curini-Galletti et at., 1985, 1989a, b; Martens et at. 1989a, b; Oki et at. 1991; Puccinelli and Curum-Galletti 1987; Tamura et at. 1991). However, the quality of chromosome preparations obtained by squashing in terms of spreading and detritus background is not as good as that obtained by using the air drying technique in other taxa. In addition, few information is available about citogenetic techniques for freshwater invertebrates. The air drying technique (Rothfelds and Siminovitch 1958) was proposed for cytogenetic analysis of mammalian bone marrow cells and it included previous tissue disgregation. For nearly forty years the technique became widely used, with several modifications, for cytogenetic studies even in plant cells. This paper describes the modifications made in the technique for the analysis of planarians chromosomes.
format Articulo
Articulo
author Margaría, Cecilia Beatriz
Dulout, Fernando Noel
author_facet Margaría, Cecilia Beatriz
Dulout, Fernando Noel
author_sort Margaría, Cecilia Beatriz
title Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
title_short Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
title_full Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
title_fullStr Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
title_full_unstemmed Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
title_sort air drying technique for cytogenetic studies in freshwater planarians (platyhelminthes)
publishDate 1997
url http://sedici.unlp.edu.ar/handle/10915/122975
work_keys_str_mv AT margariaceciliabeatriz airdryingtechniqueforcytogeneticstudiesinfreshwaterplanariansplatyhelminthes
AT duloutfernandonoel airdryingtechniqueforcytogeneticstudiesinfreshwaterplanariansplatyhelminthes
bdutipo_str Repositorios
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