Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis
Lipopolysaccharides (LPS) are the major components of the outer membrane of gram-negative bacteria. These surface molecules are relevant both for the outer membrane stability and for the interaction of the bacteria with other organisms and with the environment (18). Extensive literature is available...
Guardado en:
| Autores principales: | , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
1997
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/122765 |
| Aporte de: |
| id |
I19-R120-10915-122765 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Química Gel electrophoresis Molecular biology Chemistry Gram-negative bacteria Vibrio Polymyxin B Salmonella Ethylamines Chromatography Escherichia coli Pharmacology Electrophoresis, Polyacrylamide Gel Indicators and Reagents Rhizobium/chemistry Indicators and Reagents Lipopolysaccharides Edetic Acid triethylamine |
| spellingShingle |
Ciencias Exactas Química Gel electrophoresis Molecular biology Chemistry Gram-negative bacteria Vibrio Polymyxin B Salmonella Ethylamines Chromatography Escherichia coli Pharmacology Electrophoresis, Polyacrylamide Gel Indicators and Reagents Rhizobium/chemistry Indicators and Reagents Lipopolysaccharides Edetic Acid triethylamine Valverde, Claudio Hozbor, Daniela Flavia Lagares, Antonio Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis |
| topic_facet |
Ciencias Exactas Química Gel electrophoresis Molecular biology Chemistry Gram-negative bacteria Vibrio Polymyxin B Salmonella Ethylamines Chromatography Escherichia coli Pharmacology Electrophoresis, Polyacrylamide Gel Indicators and Reagents Rhizobium/chemistry Indicators and Reagents Lipopolysaccharides Edetic Acid triethylamine |
| description |
Lipopolysaccharides (LPS) are the major components of the outer membrane of gram-negative bacteria. These surface molecules are relevant both for the outer membrane stability and for the interaction of the bacteria with other organisms and with the environment (18). Extensive literature is available concerning LPS physiology in different symbiotic (parasitic) and pathogenic host-bacterial interaction systems (5,20). Physiological, biochemical and chemical approaches to study LPS functions commonly require LPS extraction and purification. In the literature, LPS have been prepared by a number of different cell disruption procedures. Protocols for LPS isolation include organic solvent extractions followed by exhaustive dialysis or evaporation steps, and their modifications (11,22). |
| format |
Articulo Articulo |
| author |
Valverde, Claudio Hozbor, Daniela Flavia Lagares, Antonio |
| author_facet |
Valverde, Claudio Hozbor, Daniela Flavia Lagares, Antonio |
| author_sort |
Valverde, Claudio |
| title |
Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis |
| title_short |
Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis |
| title_full |
Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis |
| title_fullStr |
Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis |
| title_full_unstemmed |
Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis |
| title_sort |
rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis |
| publishDate |
1997 |
| url |
http://sedici.unlp.edu.ar/handle/10915/122765 |
| work_keys_str_mv |
AT valverdeclaudio rapidpreparationofaffinitypurifiedlipopolysaccharidesamplesforelectrophoreticanalysis AT hozbordanielaflavia rapidpreparationofaffinitypurifiedlipopolysaccharidesamplesforelectrophoreticanalysis AT lagaresantonio rapidpreparationofaffinitypurifiedlipopolysaccharidesamplesforelectrophoreticanalysis |
| bdutipo_str |
Repositorios |
| _version_ |
1764820449182613506 |