In-house standardization and validation of a multiplex RT-PCR assay for the detection of 13 respiratory viruses

<b>Background</b>. Multiplex real time PCR is increasingly used to diagnose respiratory viruses and has shown to be superior to traditional methods, such as culture and antigen detection. <b>Objective</b>. Standardization and validation of a multiplex real-time PCR assay for...

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Autores principales: Vargas, Hernán, Diaz, Ángela, Celis, Yamile, Díaz, Liliana, Gómez, Sandra, Sánchez, Jenny, Golijow, Carlos Daniel, Arce, Patricia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Veterinaria
Multiplex Real Time PCR
Respiratory virus
Standardization
PRC múltiple en tiempo real
virus respiratorio
estandarización
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/122375
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-122375
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Multiplex Real Time PCR
Respiratory virus
Standardization
PRC múltiple en tiempo real
virus respiratorio
estandarización
spellingShingle Veterinaria
Multiplex Real Time PCR
Respiratory virus
Standardization
PRC múltiple en tiempo real
virus respiratorio
estandarización
Vargas, Hernán
Diaz, Ángela
Celis, Yamile
Díaz, Liliana
Gómez, Sandra
Sánchez, Jenny
Golijow, Carlos Daniel
Arce, Patricia
In-house standardization and validation of a multiplex RT-PCR assay for the detection of 13 respiratory viruses
topic_facet Veterinaria
Multiplex Real Time PCR
Respiratory virus
Standardization
PRC múltiple en tiempo real
virus respiratorio
estandarización
description <b>Background</b>. Multiplex real time PCR is increasingly used to diagnose respiratory viruses and has shown to be superior to traditional methods, such as culture and antigen detection. <b>Objective</b>. Standardization and validation of a multiplex real-time PCR assay for the detection of 13 respiratory viruses. <b>Methods</b>. The assay was validated using RNA control targets and comparing results to single-target PCR’s. <b>Results</b>. Using RNA controls the multiplex format was found to be as sensitive and specific as the single-target PCRs, and no competition was observed between targets. The efficiencies for most of the reactions were approximately 90%, but a lower efficiency was found for Parainfluenza 2 with a rate of amplification in each cycle of 86.63%. On the other hand, a higher efficiency was observed in respiratory syncytial virus A and respiratory syncytial virus B ((93.07% each). <b>Conclusion</b>: This multiplex RT-PCR format shows an adequate efficiency, demonstrating an excellent sensitivity, specificity and repeatability for all the studied respiratory viruses.
format Articulo
Articulo
author Vargas, Hernán
Diaz, Ángela
Celis, Yamile
Díaz, Liliana
Gómez, Sandra
Sánchez, Jenny
Golijow, Carlos Daniel
Arce, Patricia
author_facet Vargas, Hernán
Diaz, Ángela
Celis, Yamile
Díaz, Liliana
Gómez, Sandra
Sánchez, Jenny
Golijow, Carlos Daniel
Arce, Patricia
author_sort Vargas, Hernán
title In-house standardization and validation of a multiplex RT-PCR assay for the detection of 13 respiratory viruses
title_short In-house standardization and validation of a multiplex RT-PCR assay for the detection of 13 respiratory viruses
title_full In-house standardization and validation of a multiplex RT-PCR assay for the detection of 13 respiratory viruses
title_fullStr In-house standardization and validation of a multiplex RT-PCR assay for the detection of 13 respiratory viruses
title_full_unstemmed In-house standardization and validation of a multiplex RT-PCR assay for the detection of 13 respiratory viruses
title_sort in-house standardization and validation of a multiplex rt-pcr assay for the detection of 13 respiratory viruses
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/122375
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