Nuclear localization of MUC1 extracellular domain in breast, head and neck, and colon cancer

Background: The glycoprotein MUC1 is overexpressed and underglycosylated in cancer cells. MUC1 is translated as a single polypeptide that undergoes autocleavage into 2 subunits (the extracellular domain and the cytoplasmic tail), and forms a stable heterodimer at the apical membrane of normal epithe...

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Autores principales: Rabassa, Martín Enrique, Isla Larrain, Marina Teresita, Lacunza, Ezequiel, Cermignani, Luciano, Alberdi, Cecilio G., Demichelis, Sandra O., Abba, Martín Carlos, Segal Eiras, Amada, Croce, María Virginia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Médicas
MUC1 extracellular domain
Neoplasia
Nuclear localization
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/120540
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-120540
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
MUC1 extracellular domain
Neoplasia
Nuclear localization
spellingShingle Ciencias Médicas
MUC1 extracellular domain
Neoplasia
Nuclear localization
Rabassa, Martín Enrique
Isla Larrain, Marina Teresita
Lacunza, Ezequiel
Cermignani, Luciano
Alberdi, Cecilio G.
Demichelis, Sandra O.
Abba, Martín Carlos
Segal Eiras, Amada
Croce, María Virginia
Nuclear localization of MUC1 extracellular domain in breast, head and neck, and colon cancer
topic_facet Ciencias Médicas
MUC1 extracellular domain
Neoplasia
Nuclear localization
description Background: The glycoprotein MUC1 is overexpressed and underglycosylated in cancer cells. MUC1 is translated as a single polypeptide that undergoes autocleavage into 2 subunits (the extracellular domain and the cytoplasmic tail), and forms a stable heterodimer at the apical membrane of normal epithelial cells. The MUC1 cytoplasmic tail localizes to the cytoplasm of transformed cells and is targeted to the nucleus. Aims: To study the expression of the MUC1 extracellular subunit in cell nuclei of neoplastic breast, head and neck, and colon samples. Materials and methods: 330 primary tumor samples were analyzed: 166 invasive breast carcinomas, 127 head and neck tumors, and 47 colon tumors; 10 benign breast disease (BBD) and 40 normal specimens were also included. A standard immunohistochemical method with antigen retrieval was performed. Nuclear fractions from tissue homogenates and breast cancer cell lines (ZR-75, MDA-MB-231, MCF7, and T47D) were obtained and analyzed by Western blotting (WB). The anti-MUC1 extracellular subunit monoclonal antibody HMFG1 was used for immunohistochemistry. Results: 37/166 breast cancer specimens, 5/127 head and neck cancer specimens, 2/47 colon cancer samples, and 3/10 BBD samples showed immunohistochemical staining at the nuclear level. No nuclear reaction was detected in normal samples. By WB, breast and colon cancer purified nuclear fractions showed reactivity at 200 kDa in 3/30 breast and 3/20 colon cancer samples as well as purified nuclear fractions obtained from breast cancer cell lines. Conclusions: This study shows that the MUC1 extracellular domain might be translocated to the cell nucleus in breast, head and neck, and colon cancer as well as BBD.
format Articulo
Articulo
author Rabassa, Martín Enrique
Isla Larrain, Marina Teresita
Lacunza, Ezequiel
Cermignani, Luciano
Alberdi, Cecilio G.
Demichelis, Sandra O.
Abba, Martín Carlos
Segal Eiras, Amada
Croce, María Virginia
author_facet Rabassa, Martín Enrique
Isla Larrain, Marina Teresita
Lacunza, Ezequiel
Cermignani, Luciano
Alberdi, Cecilio G.
Demichelis, Sandra O.
Abba, Martín Carlos
Segal Eiras, Amada
Croce, María Virginia
author_sort Rabassa, Martín Enrique
title Nuclear localization of MUC1 extracellular domain in breast, head and neck, and colon cancer
title_short Nuclear localization of MUC1 extracellular domain in breast, head and neck, and colon cancer
title_full Nuclear localization of MUC1 extracellular domain in breast, head and neck, and colon cancer
title_fullStr Nuclear localization of MUC1 extracellular domain in breast, head and neck, and colon cancer
title_full_unstemmed Nuclear localization of MUC1 extracellular domain in breast, head and neck, and colon cancer
title_sort nuclear localization of muc1 extracellular domain in breast, head and neck, and colon cancer
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/120540
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