Purification and properties of the very high density lipoprotein from the hemolymph of adult Triatoma infestans

The very high density lipoprotein (VHDL) of Triatoma injesfum hemolymph from adult males has been isolated and purified by two-step density gradient ultracentrifugation. It appears to be homogeneous as judged by native polyacrylamide gel electrophoresis. The content of VHDL in hemolymph was estimate...

Descripción completa

Detalles Bibliográficos
Autores principales: Rimoldi, Omar Jorge, Soulages, José L., González, S. M., Peluffo, R. O., Brenner, Rodolfo Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 1989
Materias:
Biología
Insect lipoproteins
Free fatty acid trasnport
Insect VHDL
Lipid-protein interactions
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/120429
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-120429
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Insect lipoproteins
Free fatty acid trasnport
Insect VHDL
Lipid-protein interactions
spellingShingle Biología
Insect lipoproteins
Free fatty acid trasnport
Insect VHDL
Lipid-protein interactions
Rimoldi, Omar Jorge
Soulages, José L.
González, S. M.
Peluffo, R. O.
Brenner, Rodolfo Roberto
Purification and properties of the very high density lipoprotein from the hemolymph of adult Triatoma infestans
topic_facet Biología
Insect lipoproteins
Free fatty acid trasnport
Insect VHDL
Lipid-protein interactions
description The very high density lipoprotein (VHDL) of Triatoma injesfum hemolymph from adult males has been isolated and purified by two-step density gradient ultracentrifugation. It appears to be homogeneous as judged by native polyacrylamide gel electrophoresis. The content of VHDL in hemolymph was estimated to be 8 mg proteidml. The purified protein has a molecular weight (M,) of 450,000, is composed of six subunits of M, p 77,000, and possesses a high content of aromatic amino acids. This protein is glycosylated and contains 3% of lipids by weight with a remarkable amount of free fatty acids (25% of total lipids). The I: injesfans VHDL has a different lipid and amino acid composition from lipophorin. The lipid composition and the spectroscopic studies using cis-parinaric acid indicated a high fatty acid binding affinity. It has nine binding sites per mol of VHDL. Competence studies revealed that VHDL has its highest affinity for the binding of palmitic acid followed by stearic and arachidonic acids.
format Articulo
Articulo
author Rimoldi, Omar Jorge
Soulages, José L.
González, S. M.
Peluffo, R. O.
Brenner, Rodolfo Roberto
author_facet Rimoldi, Omar Jorge
Soulages, José L.
González, S. M.
Peluffo, R. O.
Brenner, Rodolfo Roberto
author_sort Rimoldi, Omar Jorge
title Purification and properties of the very high density lipoprotein from the hemolymph of adult Triatoma infestans
title_short Purification and properties of the very high density lipoprotein from the hemolymph of adult Triatoma infestans
title_full Purification and properties of the very high density lipoprotein from the hemolymph of adult Triatoma infestans
title_fullStr Purification and properties of the very high density lipoprotein from the hemolymph of adult Triatoma infestans
title_full_unstemmed Purification and properties of the very high density lipoprotein from the hemolymph of adult Triatoma infestans
title_sort purification and properties of the very high density lipoprotein from the hemolymph of adult triatoma infestans
publishDate 1989
url http://sedici.unlp.edu.ar/handle/10915/120429
work_keys_str_mv AT rimoldiomarjorge purificationandpropertiesoftheveryhighdensitylipoproteinfromthehemolymphofadulttriatomainfestans
AT soulagesjosel purificationandpropertiesoftheveryhighdensitylipoproteinfromthehemolymphofadulttriatomainfestans
AT gonzalezsm purificationandpropertiesoftheveryhighdensitylipoproteinfromthehemolymphofadulttriatomainfestans
AT pelufforo purificationandpropertiesoftheveryhighdensitylipoproteinfromthehemolymphofadulttriatomainfestans
AT brennerrodolforoberto purificationandpropertiesoftheveryhighdensitylipoproteinfromthehemolymphofadulttriatomainfestans
bdutipo_str Repositorios
_version_ 1764820449917665281

Ejemplares similares