Allergenicity reduction of cow's milk proteins using latex peptidases

The present study evaluated four laticifer fluids as a novel source of peptidases capable of hydrolyzing proteins in cow’s milk. The latex peptidases from Calotropis procera (CpLP), Cryptostegia grandiflora (CgLP), and Carica papaya (CapLP) were able to perform total hydrolysis of caseins after 30 m...

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Detalles Bibliográficos
Autores principales: Oliveira, João P.B., Candreva, Ángela María, Rizzo, Gastón, Ramos, Márcio V., Oliveira, Jefferson S., Oliveira, Hermógenes D., Ary, Maria B., Docena, Guillermo Horacio, Freitas, Cleverson D.T.
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2019
Materias:
Química
Allergy
Laticifer
Protein
Protease
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/119949
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-119949
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Allergy
Laticifer
Protein
Protease
spellingShingle Química
Allergy
Laticifer
Protein
Protease
Oliveira, João P.B.
Candreva, Ángela María
Rizzo, Gastón
Ramos, Márcio V.
Oliveira, Jefferson S.
Oliveira, Hermógenes D.
Ary, Maria B.
Docena, Guillermo Horacio
Freitas, Cleverson D.T.
Allergenicity reduction of cow's milk proteins using latex peptidases
topic_facet Química
Allergy
Laticifer
Protein
Protease
description The present study evaluated four laticifer fluids as a novel source of peptidases capable of hydrolyzing proteins in cow’s milk. The latex peptidases from Calotropis procera (CpLP), Cryptostegia grandiflora (CgLP), and Carica papaya (CapLP) were able to perform total hydrolysis of caseins after 30 min at pH 6.5, as confirmed by a significant reduction in the residual antigenicity. Casein hydrolysis by Plumeria rubra latex peptidases (PrLP) was negligible. Moreover, whey proteins were more resistant to proteolysis by latex peptidases; however, heat pretreatment of the whey proteins enhanced the degree of hydrolysis and reduced the residual antigenicity of the hydrolysates. The in vivo assays show that the cow’s milk proteins hydrolysed by CgLP and CapLP exhibited no immune reactions in mice allergic to cow’s milk, similar to a commercial partially hydrolysed formula. Thus, these peptidases are promising enzymes for the development of novel hypoallergenic formulas for children with a milk allergy.
format Articulo
Preprint
author Oliveira, João P.B.
Candreva, Ángela María
Rizzo, Gastón
Ramos, Márcio V.
Oliveira, Jefferson S.
Oliveira, Hermógenes D.
Ary, Maria B.
Docena, Guillermo Horacio
Freitas, Cleverson D.T.
author_facet Oliveira, João P.B.
Candreva, Ángela María
Rizzo, Gastón
Ramos, Márcio V.
Oliveira, Jefferson S.
Oliveira, Hermógenes D.
Ary, Maria B.
Docena, Guillermo Horacio
Freitas, Cleverson D.T.
author_sort Oliveira, João P.B.
title Allergenicity reduction of cow's milk proteins using latex peptidases
title_short Allergenicity reduction of cow's milk proteins using latex peptidases
title_full Allergenicity reduction of cow's milk proteins using latex peptidases
title_fullStr Allergenicity reduction of cow's milk proteins using latex peptidases
title_full_unstemmed Allergenicity reduction of cow's milk proteins using latex peptidases
title_sort allergenicity reduction of cow's milk proteins using latex peptidases
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/119949
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