Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived From Bordetella pertussis

Outer Membrane Vesicles (OMVs) derived from different Gram-negative bacteria have been proposed as an attractive vaccine platform because of their own immunogenic adjuvant properties. Pertussis or whooping cough is a highly contagious vaccine-preventable respiratory disease that resurged during the...

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Detalles Bibliográficos
Autores principales: Elizagaray, Maia Lina, Gomes, Marco Túlio R., Guimaraes, Erika S., Rumbo, Martín, Hozbor, Daniela Flavia, Oliveira, Sergio C., Moreno, Griselda
Formato: Articulo Reporte
Lenguaje:Inglés
Publicado: 2020
Materias:
Biología
Inflammasome
Caspases 1/11
Bordetella pertussis
Outer membrane vesicles
LOS
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/119721
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-119721
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Inflammasome
Caspases 1/11
Bordetella pertussis
Outer membrane vesicles
LOS
spellingShingle Biología
Inflammasome
Caspases 1/11
Bordetella pertussis
Outer membrane vesicles
LOS
Elizagaray, Maia Lina
Gomes, Marco Túlio R.
Guimaraes, Erika S.
Rumbo, Martín
Hozbor, Daniela Flavia
Oliveira, Sergio C.
Moreno, Griselda
Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived From Bordetella pertussis
topic_facet Biología
Inflammasome
Caspases 1/11
Bordetella pertussis
Outer membrane vesicles
LOS
description Outer Membrane Vesicles (OMVs) derived from different Gram-negative bacteria have been proposed as an attractive vaccine platform because of their own immunogenic adjuvant properties. Pertussis or whooping cough is a highly contagious vaccine-preventable respiratory disease that resurged during the last decades in many countries. In response to the epidemiological situation, new boosters have been incorporated into vaccination schedules worldwide and new vaccine candidates have started to be designed. Particularly, our group designed a new pertussis vaccine candidate based on OMVs derived from Bordetella pertussis (BpOMVs). To continue with the characterization of the immune response induced by our OMV based vaccine candidate, this work aimed to investigate the ability of OMVs to activate the inflammasome pathway in macrophages. We observed that NLRP3, caspase-1/11, and gasdermin-D (GSDMD) are involved in inflammasome activation by BpOMVs. Moreover, we demonstrated that BpOMVs as well as transfected B. pertussis lipooligosaccharide (BpLOS) induce caspase-11 (Casp11) and guanylate-binding proteins (GBPs) dependent non-canonical inflammasome activation. Our results elucidate the mechanism by which BpOMVs trigger one central pathway of the innate response activation that is expected to skew the adaptive immune response elicited by BpOMVs vaccination.
format Articulo
Reporte
author Elizagaray, Maia Lina
Gomes, Marco Túlio R.
Guimaraes, Erika S.
Rumbo, Martín
Hozbor, Daniela Flavia
Oliveira, Sergio C.
Moreno, Griselda
author_facet Elizagaray, Maia Lina
Gomes, Marco Túlio R.
Guimaraes, Erika S.
Rumbo, Martín
Hozbor, Daniela Flavia
Oliveira, Sergio C.
Moreno, Griselda
author_sort Elizagaray, Maia Lina
title Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived From Bordetella pertussis
title_short Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived From Bordetella pertussis
title_full Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived From Bordetella pertussis
title_fullStr Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived From Bordetella pertussis
title_full_unstemmed Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived From Bordetella pertussis
title_sort canonical and non-canonical inflammasome activation by outer membrane vesicles derived from bordetella pertussis
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/119721
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