Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III- and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Imazethapyr (IMZT) is a selective postemergent herbicide with residual action. Available data analyzing its effects in aquatic vertebrates are scarce. In previous studies, we demonstrated that IMZT induces lesions into the DNA of Hypsiboas pulchellus tadpoles using the single-cell gel electrophoresi...

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Detalles Bibliográficos
Autores principales:	Pérez Iglesias, Juan Manuel, Ruiz de Arcaute, Celeste, Natale, Guillermo Sebastián, Soloneski, Sonia María Elsa, Larramendy, Marcelo Luis
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2017
Materias:	Química Biología Endo III Fpg Herbicide Imazethapyr-based formulation Modified comet assay Oxidative DNA damage
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/119254
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-119254
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Química Biología Endo III Fpg Herbicide Imazethapyr-based formulation Modified comet assay Oxidative DNA damage
spellingShingle	Química Biología Endo III Fpg Herbicide Imazethapyr-based formulation Modified comet assay Oxidative DNA damage Pérez Iglesias, Juan Manuel Ruiz de Arcaute, Celeste Natale, Guillermo Sebastián Soloneski, Sonia María Elsa Larramendy, Marcelo Luis Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III- and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles (Anura, Hylidae)
topic_facet	Química Biología Endo III Fpg Herbicide Imazethapyr-based formulation Modified comet assay Oxidative DNA damage
description	Imazethapyr (IMZT) is a selective postemergent herbicide with residual action. Available data analyzing its effects in aquatic vertebrates are scarce. In previous studies, we demonstrated that IMZT induces lesions into the DNA of Hypsiboas pulchellus tadpoles using the single-cell gel electrophoresis (SCGE) assay as a biomarker for genotoxicity. Currently, this assay can be modified by including incubation with lesion-specific endonucleases, e.g., endonuclease III (Endo III) and formamidopyrimidine-DNA glycosylase (Fpg), which detect oxidized pyrimidine and purine bases, respectively. The aim of this study was to evaluate the role of oxidative stress in the genotoxic damage in circulating blood cells of H. pulchellus tadpoles exposed to the IMZT-based Pivot H® formulation (10.59% IMZT) at a concentration equivalent to 25% of the LC50 (96 h) value (0.39 mg/L IMZT) during 48 and 96 h. Our results demonstrate that the herbicide induces oxidative DNA damage on H. pulchellus tadpoles at purines bases but not at pyrimidines. Our findings represent the first evidence of oxidative damage caused by IMZT on anuran DNA using the alkaline restriction enzyme-modified SCGE assay.
format	Articulo Preprint
author	Pérez Iglesias, Juan Manuel Ruiz de Arcaute, Celeste Natale, Guillermo Sebastián Soloneski, Sonia María Elsa Larramendy, Marcelo Luis
author_facet	Pérez Iglesias, Juan Manuel Ruiz de Arcaute, Celeste Natale, Guillermo Sebastián Soloneski, Sonia María Elsa Larramendy, Marcelo Luis
author_sort	Pérez Iglesias, Juan Manuel
title	Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III- and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles (Anura, Hylidae)
title_short	Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III- and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles (Anura, Hylidae)
title_full	Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III- and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles (Anura, Hylidae)
title_fullStr	Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III- and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles (Anura, Hylidae)
title_full_unstemmed	Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III- and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles (Anura, Hylidae)
title_sort	evaluation of imazethapyr-induced dna oxidative damage by alkaline endo iii- and fpg-modified single-cell gel electrophoresis assay in hypsiboas pulchellus tadpoles (anura, hylidae)
publishDate	2017
url	http://sedici.unlp.edu.ar/handle/10915/119254
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bdutipo_str	Repositorios
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Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III- and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles (Anura, Hylidae)

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