Purification and characterization of two inducible exopolygalacturonases from Aspergillus kawachii

Of two exopolygalacturonases purified and characterized from an Aspergillus kawachii culture grown on lemon pomace, the main one, exoPG1, was a glycosylated protein with a molecular mass of 75 kDa, isoelectric point in the 4.00–4.65 pH range, and a 3.0–4.0 pH optimum, though with activity at pH 2.0....

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Autores principales: Byrne, Christian Eduardo, Cavalitto, Sebastián Fernando, Voget, Claudio Enrique
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2017
Materias:
Química
Aspergillus kawachii
Exopolygalacturonases
Pectin degradation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/119250
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-119250
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Aspergillus kawachii
Exopolygalacturonases
Pectin degradation
spellingShingle Química
Aspergillus kawachii
Exopolygalacturonases
Pectin degradation
Byrne, Christian Eduardo
Cavalitto, Sebastián Fernando
Voget, Claudio Enrique
Purification and characterization of two inducible exopolygalacturonases from Aspergillus kawachii
topic_facet Química
Aspergillus kawachii
Exopolygalacturonases
Pectin degradation
description Of two exopolygalacturonases purified and characterized from an Aspergillus kawachii culture grown on lemon pomace, the main one, exoPG1, was a glycosylated protein with a molecular mass of 75 kDa, isoelectric point in the 4.00–4.65 pH range, and a 3.0–4.0 pH optimum, though with activity at pH 2.0. ExoPG1 cleaved monomer units irrespective of the degree of substrate polymerization. Di- and trigalacturonic acids were completely hydrolyzed, whereas polygalacturonic acid (PGA) only incompletely. ExoPG1, along with a recombinant endoPG from the same fungal strain, was necessary for the hydrolysis of PGA down to the monomer. pH stability was maximum in the range 4.0–5.0 irrespective of the incubation temperature and decreased as the temperature increased from 30 to 70 °C. The enzyme appeared not to require divalent cations for activity. Protein identification by MALDI-TOF-TOF MS/MS indicated homology of exoPG1 with the exopolygalacturonase PGXB of Aspergillus. niger, an exopolygalacturonase of Aspergillus tubingensis, and the exopolygalacturonase X of Aspergillus kawachii, a hypothetical enzyme predicted from the complete sequencing of the genome of the fungus. Both these latter proteins are unusual in that they have identical primary sequences. We therefore conclude that exoPG1 is probably the hypothetical A. kawachii exopolygalacturonase X. ExoPG2—having a molecular weight of 80 kDa, an isoelectricpoint between pHs 4.5 and 5.0, a 4.0 pH optimum, and kinetics with PGA similar to those of exoPG1—shared similarities with the exopolygalacturonase PGXC of A. niger and another proposed exopolygalacturonase of A. kawachii. This report is the first concerning exopolygalacturonases from A. kawachii.
format Articulo
Preprint
author Byrne, Christian Eduardo
Cavalitto, Sebastián Fernando
Voget, Claudio Enrique
author_facet Byrne, Christian Eduardo
Cavalitto, Sebastián Fernando
Voget, Claudio Enrique
author_sort Byrne, Christian Eduardo
title Purification and characterization of two inducible exopolygalacturonases from Aspergillus kawachii
title_short Purification and characterization of two inducible exopolygalacturonases from Aspergillus kawachii
title_full Purification and characterization of two inducible exopolygalacturonases from Aspergillus kawachii
title_fullStr Purification and characterization of two inducible exopolygalacturonases from Aspergillus kawachii
title_full_unstemmed Purification and characterization of two inducible exopolygalacturonases from Aspergillus kawachii
title_sort purification and characterization of two inducible exopolygalacturonases from aspergillus kawachii
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/119250
work_keys_str_mv AT byrnechristianeduardo purificationandcharacterizationoftwoinducibleexopolygalacturonasesfromaspergilluskawachii
AT cavalittosebastianfernando purificationandcharacterizationoftwoinducibleexopolygalacturonasesfromaspergilluskawachii
AT vogetclaudioenrique purificationandcharacterizationoftwoinducibleexopolygalacturonasesfromaspergilluskawachii
bdutipo_str Repositorios
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