Isolation of Beauveria bassiana from the Chagas Disease Vector Triatoma infestans in the Gran Chaco Region of Argentina: Assessment of Gene Expression during Host–Pathogen Interaction

A native strain of the entomopathogenic fungus Beauveria bassiana (Bb-C001) was isolated from a naturally infected Triatoma infestans, Klug (Hemiptera: Reduviidae) adult cadaver in the Gran Chaco region, Salta province, Argentina. The isolate was both phenotypic and molecularly characterized in a co...

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Autores principales: Baldiviezo, Linda Vanesa, Pedrini, Nicolás, Santana, Marianela, Mannino, María Constanza, Nieva, Lucía Beatriz, Gentile, Alberto, Cardozo, Rubén Marino
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Bioquímica
Entomopathogenic fungi
Resistant triatomines
Biological control
Bassianolide
Beauvericin
Limpet
Dual gene expression
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/118939
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-118939
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Entomopathogenic fungi
Resistant triatomines
Biological control
Bassianolide
Beauvericin
Limpet
Dual gene expression
spellingShingle Bioquímica
Entomopathogenic fungi
Resistant triatomines
Biological control
Bassianolide
Beauvericin
Limpet
Dual gene expression
Baldiviezo, Linda Vanesa
Pedrini, Nicolás
Santana, Marianela
Mannino, María Constanza
Nieva, Lucía Beatriz
Gentile, Alberto
Cardozo, Rubén Marino
Isolation of Beauveria bassiana from the Chagas Disease Vector Triatoma infestans in the Gran Chaco Region of Argentina: Assessment of Gene Expression during Host–Pathogen Interaction
topic_facet Bioquímica
Entomopathogenic fungi
Resistant triatomines
Biological control
Bassianolide
Beauvericin
Limpet
Dual gene expression
description A native strain of the entomopathogenic fungus Beauveria bassiana (Bb-C001) was isolated from a naturally infected Triatoma infestans, Klug (Hemiptera: Reduviidae) adult cadaver in the Gran Chaco region, Salta province, Argentina. The isolate was both phenotypic and molecularly characterized in a context of fungus-insect interaction, by measuring the expression pattern of toxin genes during infection and immune response of T. infestans. The commercial strain GHA of B. bassiana, which was previously used in field interventions to control these vectors, was used as reference in this study. The phylogenetic trees based on both ribosomal internal transcribed spacer (ITS) and elongation factor 1-alpha (EF1-α) indicated that Bb-C001 fits into a B. bassiana cluster, and the sequence-characterized amplified regions (SCAR) showed that Bb-C001 is different from the GHA strain. There were no differences between both strains regarding viability, radial growth, and conidia production, either in the median survival time or insect mortality. However, Bb-C001 showed a higher expression than GHA of the bassianolide synthetase gene (BbbslS) during infection, and similar levels of the beauvericin synthetase gene (BbbeaS). Immune-related genes of T. infestans nymphs (limpet-2 and defensin-1, -2, and -6) were later expressed and thus insects failed to stop the infection process. These results showed that B. bassiana Bb-C001 is a promised fungal strain to be incorporated in the current biological control programs of T. infestans in Salta province, Argentina.
format Articulo
Articulo
author Baldiviezo, Linda Vanesa
Pedrini, Nicolás
Santana, Marianela
Mannino, María Constanza
Nieva, Lucía Beatriz
Gentile, Alberto
Cardozo, Rubén Marino
author_facet Baldiviezo, Linda Vanesa
Pedrini, Nicolás
Santana, Marianela
Mannino, María Constanza
Nieva, Lucía Beatriz
Gentile, Alberto
Cardozo, Rubén Marino
author_sort Baldiviezo, Linda Vanesa
title Isolation of Beauveria bassiana from the Chagas Disease Vector Triatoma infestans in the Gran Chaco Region of Argentina: Assessment of Gene Expression during Host–Pathogen Interaction
title_short Isolation of Beauveria bassiana from the Chagas Disease Vector Triatoma infestans in the Gran Chaco Region of Argentina: Assessment of Gene Expression during Host–Pathogen Interaction
title_full Isolation of Beauveria bassiana from the Chagas Disease Vector Triatoma infestans in the Gran Chaco Region of Argentina: Assessment of Gene Expression during Host–Pathogen Interaction
title_fullStr Isolation of Beauveria bassiana from the Chagas Disease Vector Triatoma infestans in the Gran Chaco Region of Argentina: Assessment of Gene Expression during Host–Pathogen Interaction
title_full_unstemmed Isolation of Beauveria bassiana from the Chagas Disease Vector Triatoma infestans in the Gran Chaco Region of Argentina: Assessment of Gene Expression during Host–Pathogen Interaction
title_sort isolation of beauveria bassiana from the chagas disease vector triatoma infestans in the gran chaco region of argentina: assessment of gene expression during host–pathogen interaction
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/118939
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