LINC00885 a Novel Oncogenic Long Non-Coding RNA Associated with Early Stage Breast Cancer Progression

Long intergenic non-protein coding RNA 885 (LINC00885) was identified as significantly upregulated in breast ductal carcinoma in situ (DCIS). The aim of this study was to characterize the phenotypic e ects and signaling pathways modulated by LINC00885 in non-invasive and invasive breast cancer model...

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Autores principales: Abba, Martín Carlos, Canzoneri, Romina, Gurruchaga, Agustina, Lee, Jaeho, Tatineni, Pradeep, Kil, Hyunsuk, Lacunza, Ezequiel, Aldaz, C. Marcelo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Médicas
LINC00885
lncRNA
Breast cancer
DCIS
Proliferation
Invasion
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/118936
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-118936
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
LINC00885
lncRNA
Breast cancer
DCIS
Proliferation
Invasion
spellingShingle Ciencias Médicas
LINC00885
lncRNA
Breast cancer
DCIS
Proliferation
Invasion
Abba, Martín Carlos
Canzoneri, Romina
Gurruchaga, Agustina
Lee, Jaeho
Tatineni, Pradeep
Kil, Hyunsuk
Lacunza, Ezequiel
Aldaz, C. Marcelo
LINC00885 a Novel Oncogenic Long Non-Coding RNA Associated with Early Stage Breast Cancer Progression
topic_facet Ciencias Médicas
LINC00885
lncRNA
Breast cancer
DCIS
Proliferation
Invasion
description Long intergenic non-protein coding RNA 885 (LINC00885) was identified as significantly upregulated in breast ductal carcinoma in situ (DCIS). The aim of this study was to characterize the phenotypic e ects and signaling pathways modulated by LINC00885 in non-invasive and invasive breast cancer models. We determined that LINC00885 induces premalignant phenotypic changes by increasing cell proliferation, motility, migration and altering 3D growth in normal and DCIS breast cell lines. Transcriptomic studies (RNA-seq) identified the main signaling pathways modulated by LINC00885, which include bioprocesses related to TP53 signaling pathway and proliferative signatures such as activation of EREG, EGFR and FOXM1 pathways. LINC00885 silencing in breast cancer lines overexpressing this lncRNA leads to downregulation of proliferation related transcripts such as EREG, CMYC, CCND1 and to significant decrease in cell migration and motility. TCGA-BRCA data analyses show an association between high LINC00885 expression and worse overall survival in patients with primary invasive breast carcinomas (p = 0.024), suggesting that the pro-tumorigenic e ects of LINC00885 overexpression persist post-invasion. We conclude that LINC00885 behaves as a positive regulator of cell growth both in normal and DCIS breast cells possibly operating as a ceRNA and representing a novel oncogenic lncRNA associated with early stage breast cancer progression.
format Articulo
Articulo
author Abba, Martín Carlos
Canzoneri, Romina
Gurruchaga, Agustina
Lee, Jaeho
Tatineni, Pradeep
Kil, Hyunsuk
Lacunza, Ezequiel
Aldaz, C. Marcelo
author_facet Abba, Martín Carlos
Canzoneri, Romina
Gurruchaga, Agustina
Lee, Jaeho
Tatineni, Pradeep
Kil, Hyunsuk
Lacunza, Ezequiel
Aldaz, C. Marcelo
author_sort Abba, Martín Carlos
title LINC00885 a Novel Oncogenic Long Non-Coding RNA Associated with Early Stage Breast Cancer Progression
title_short LINC00885 a Novel Oncogenic Long Non-Coding RNA Associated with Early Stage Breast Cancer Progression
title_full LINC00885 a Novel Oncogenic Long Non-Coding RNA Associated with Early Stage Breast Cancer Progression
title_fullStr LINC00885 a Novel Oncogenic Long Non-Coding RNA Associated with Early Stage Breast Cancer Progression
title_full_unstemmed LINC00885 a Novel Oncogenic Long Non-Coding RNA Associated with Early Stage Breast Cancer Progression
title_sort linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/118936
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