Anti-tumoral Effect of a Cell Penetrating and Interfering Peptide Targeting PP2A/SET Interaction

Objective: To test cell penetrating and interfering peptide Mut3DPT-PP2A/SET in interaction between serine threonine phosphatase PP2A and its physiological inhibitor, the oncoprotein SET. Materials and methods: Adult male C3H/S-strain mice, 60 days old, were given a graft of breast adenocarcinoma c...

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Detalles Bibliográficos
Autores principales: Andrini, Laura Beatriz, Marín, Gustavo Horacio, Inda, Ana María, Bruzzoni Giovanelli, Heriberto, García, Marcela Nilda, Errecalde, Jorge Oscar, Rebollo, Angelita
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Médicas
Apoptosis
Cáncer
Drug research
Peptide
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/118932
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-118932
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Apoptosis
Cáncer
Drug research
Peptide
spellingShingle Ciencias Médicas
Apoptosis
Cáncer
Drug research
Peptide
Andrini, Laura Beatriz
Marín, Gustavo Horacio
Inda, Ana María
Bruzzoni Giovanelli, Heriberto
García, Marcela Nilda
Errecalde, Jorge Oscar
Rebollo, Angelita
Anti-tumoral Effect of a Cell Penetrating and Interfering Peptide Targeting PP2A/SET Interaction
topic_facet Ciencias Médicas
Apoptosis
Cáncer
Drug research
Peptide
description Objective: To test cell penetrating and interfering peptide Mut3DPT-PP2A/SET in interaction between serine threonine phosphatase PP2A and its physiological inhibitor, the oncoprotein SET. Materials and methods: Adult male C3H/S-strain mice, 60 days old, were given a graft of breast adenocarcinoma cells (TN60) into subcutaneous tissue. Mut3DPT-PP2A/SET peptide was used to block PP2A and SET oncoprotein interaction. The graft-bearing animals were divided into a control group (injected with saline buffer), and an intervention group injected intraperitoneally with Mut3DPTPP2A/ SET peptide (5 mg/kg) every day from day 5 to day 37. The variables we used to compare the outcome in both groups were tumor size in mm (length×width) and histological changes. In the statistical analysis we used ANOVA and Student-Keuls multiple comparisons test and Tuckey for the post-test analysis. Results: 48 mice were grafted at day 0 with breast UNLP-C3H/S tumor cells, and after randomization, they were assigned to one of the two study groups. At day 5 all mice were injected either with placebo or with the peptide. The treated group showed significant tumor reduction (p<0.07). Histological changes showed presence of apoptosis and necrosis of tumor in treated group. Conclusion: The peptide Mut3DPT-PP2A/SET has demonstrated anti-tumor activity by reduction in vivo of tumor growth becoming a promising future in anticancer therapy.
format Articulo
Articulo
author Andrini, Laura Beatriz
Marín, Gustavo Horacio
Inda, Ana María
Bruzzoni Giovanelli, Heriberto
García, Marcela Nilda
Errecalde, Jorge Oscar
Rebollo, Angelita
author_facet Andrini, Laura Beatriz
Marín, Gustavo Horacio
Inda, Ana María
Bruzzoni Giovanelli, Heriberto
García, Marcela Nilda
Errecalde, Jorge Oscar
Rebollo, Angelita
author_sort Andrini, Laura Beatriz
title Anti-tumoral Effect of a Cell Penetrating and Interfering Peptide Targeting PP2A/SET Interaction
title_short Anti-tumoral Effect of a Cell Penetrating and Interfering Peptide Targeting PP2A/SET Interaction
title_full Anti-tumoral Effect of a Cell Penetrating and Interfering Peptide Targeting PP2A/SET Interaction
title_fullStr Anti-tumoral Effect of a Cell Penetrating and Interfering Peptide Targeting PP2A/SET Interaction
title_full_unstemmed Anti-tumoral Effect of a Cell Penetrating and Interfering Peptide Targeting PP2A/SET Interaction
title_sort anti-tumoral effect of a cell penetrating and interfering peptide targeting pp2a/set interaction
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/118932
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