Mucosal Immunoregulatory Properties of Tsukamurella inchonensis to Reverse Experimental Food Allergy

The intestinal mucosa is lined by epithelial cells, which are key cells to sustain gut homeostasis. Food allergy is an immune-mediated adverse reaction to food, likely due to defective regulatory circuits. Tsukamurella inchonensis is a non-pathogenic bacterium with immunomodulatory properties. We hy...

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Detalles Bibliográficos
Autores principales:	Smaldini, Paola Lorena, Trejo, Fernando Miguel, Rizzo, Gastón, Comerci, Diego J., Kampinga, Jaap, Docena, Guillermo Horacio
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2021
Materias:	Biología Tsukamurella inchonensis Food hypersensitivity Anti-inflammatory agents Enterocytes Intestinal mucosa
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/118862
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-118862
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Tsukamurella inchonensis Food hypersensitivity Anti-inflammatory agents Enterocytes Intestinal mucosa
spellingShingle	Biología Tsukamurella inchonensis Food hypersensitivity Anti-inflammatory agents Enterocytes Intestinal mucosa Smaldini, Paola Lorena Trejo, Fernando Miguel Rizzo, Gastón Comerci, Diego J. Kampinga, Jaap Docena, Guillermo Horacio Mucosal Immunoregulatory Properties of Tsukamurella inchonensis to Reverse Experimental Food Allergy
topic_facet	Biología Tsukamurella inchonensis Food hypersensitivity Anti-inflammatory agents Enterocytes Intestinal mucosa
description	The intestinal mucosa is lined by epithelial cells, which are key cells to sustain gut homeostasis. Food allergy is an immune-mediated adverse reaction to food, likely due to defective regulatory circuits. Tsukamurella inchonensis is a non-pathogenic bacterium with immunomodulatory properties. We hypothesize that the anti-inflammatory effect of dead T. inchonensis on activated epithelial cells modulates milk allergy through the restoration of tolerance in a mouse model. Epithelial cells (Caco-2 and enterocytes from mouse gut) and macrophages were stimulated with T. inchonensis and induction of luciferase under the NF-kB promoter, ROS and cytokines production were studied. Balb/cmice weremucosally sensitized with cow´s milk proteins plus cholera toxin and orally challenged with the allergen to evidence hypersensitivity symptoms. After that, mice were orally administered with heatkilled T. inchonensis as treatment and then challenged with the allergen. The therapeutic efficacy was in vivo (clinical score and cutaneous test) and in vitro (serum specific antibodies and cytokines-ELISA, and cell analysis-flow cytometry) evaluated. Heat-killed T. inchonensis modulated the induction of pro-inflammatory chemokines, with an increase in antiinflammatory cytokines by intestinal epithelial cells and by macrophages with decreased OX40L expression. In vivo, oral administration of T. inchonensis increased the frequency of lamina propria CD4+CD25+FoxP3+ T cells, and clinical signs were lower in T. inchonensistreated mice compared with milk-sensitized animals. In vivo depletion of Tregs (anti-CD25) abrogated T. inchonensis immunomodulation. In conclusion, these bacteria suppressed the intestinal inflammatory immune response to reverse food allergy.
format	Articulo Articulo
author	Smaldini, Paola Lorena Trejo, Fernando Miguel Rizzo, Gastón Comerci, Diego J. Kampinga, Jaap Docena, Guillermo Horacio
author_facet	Smaldini, Paola Lorena Trejo, Fernando Miguel Rizzo, Gastón Comerci, Diego J. Kampinga, Jaap Docena, Guillermo Horacio
author_sort	Smaldini, Paola Lorena
title	Mucosal Immunoregulatory Properties of Tsukamurella inchonensis to Reverse Experimental Food Allergy
title_short	Mucosal Immunoregulatory Properties of Tsukamurella inchonensis to Reverse Experimental Food Allergy
title_full	Mucosal Immunoregulatory Properties of Tsukamurella inchonensis to Reverse Experimental Food Allergy
title_fullStr	Mucosal Immunoregulatory Properties of Tsukamurella inchonensis to Reverse Experimental Food Allergy
title_full_unstemmed	Mucosal Immunoregulatory Properties of Tsukamurella inchonensis to Reverse Experimental Food Allergy
title_sort	mucosal immunoregulatory properties of tsukamurella inchonensis to reverse experimental food allergy
publishDate	2021
url	http://sedici.unlp.edu.ar/handle/10915/118862
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bdutipo_str	Repositorios
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Mucosal Immunoregulatory Properties of Tsukamurella inchonensis to Reverse Experimental Food Allergy

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