In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis
Antigen formulation is the main feature for the success of leishmaniosis diagnosis and vaccination, since the disease is caused by different parasite species that display particularities which determine their pathogenicity and virulence. It is desirable that the antigens are recognized by different...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2021
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/118860 |
| Aporte de: |
| id |
I19-R120-10915-118860 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Química Mimetic peptides Cytokines In vitro infection Vaccines Leishmaniasis |
| spellingShingle |
Química Mimetic peptides Cytokines In vitro infection Vaccines Leishmaniasis Carbonera Guedes, Deborah Hospinal Santiani, Manuel Carvalho, Joyce Soccol, Carlos Ricardo Minozzo, João Carlos Machado de Ávila, Ricardo Andrez Ferreira de Moura, Juliana Pires Ramos, Eliezer Lucas Castro, Guillermo Raúl Chávez-Olórtegi, Carlos Thomaz-Soccol, Vanete In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis |
| topic_facet |
Química Mimetic peptides Cytokines In vitro infection Vaccines Leishmaniasis |
| description |
Antigen formulation is the main feature for the success of leishmaniosis diagnosis and vaccination, since the disease is caused by different parasite species that display particularities which determine their pathogenicity and virulence. It is desirable that the antigens are recognized by different antibodies and are immunogenic for almost all Leishmania species. To overcome this problem, we selected six potentially immunogenic peptides derived from Leishmania histones and parasite membrane molecules obtained by phage display or spot synthesis and entrapped in liposome structures.We used these peptides to immunize New Zealand rabbits and determine the immunogenic capacity of the chimeric antigen. The peptides induced the production of antibodies as a humoral immune response against L. braziliensis or L. infantum. Next, to evaluate the innate response to induce cellular activation, macrophages from the peptide mix-immunized rabbits were infected in vitro with L. braziliensis or L. infantum. The peptidemix generated the IFN-g, IL-12, IL-4 and TGF-b that led to Th1 and Th2 cellular immune responses.
Interestingly, this mix of peptides also induced high expression of iNOS. These results suggest that themix of peptides derived fromhistone and parasitesmembranemolecules was able to mimic parasites proteins and induce cytokines important to CD4+ T cell Th1 and Th2 differentiation and effector molecule to control the parasite infection. Finally, this peptide induced an immune balance that is important to prevent immunopathological disorders, inflammatory reactions, and control the parasite infection. |
| format |
Articulo Articulo |
| author |
Carbonera Guedes, Deborah Hospinal Santiani, Manuel Carvalho, Joyce Soccol, Carlos Ricardo Minozzo, João Carlos Machado de Ávila, Ricardo Andrez Ferreira de Moura, Juliana Pires Ramos, Eliezer Lucas Castro, Guillermo Raúl Chávez-Olórtegi, Carlos Thomaz-Soccol, Vanete |
| author_facet |
Carbonera Guedes, Deborah Hospinal Santiani, Manuel Carvalho, Joyce Soccol, Carlos Ricardo Minozzo, João Carlos Machado de Ávila, Ricardo Andrez Ferreira de Moura, Juliana Pires Ramos, Eliezer Lucas Castro, Guillermo Raúl Chávez-Olórtegi, Carlos Thomaz-Soccol, Vanete |
| author_sort |
Carbonera Guedes, Deborah |
| title |
In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis |
| title_short |
In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis |
| title_full |
In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis |
| title_fullStr |
In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis |
| title_full_unstemmed |
In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis |
| title_sort |
in silico and in vitro evaluation of mimetic peptides as potential antigen candidates for prophylaxis of leishmaniosis |
| publishDate |
2021 |
| url |
http://sedici.unlp.edu.ar/handle/10915/118860 |
| work_keys_str_mv |
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Repositorios |
| _version_ |
1764820447645401093 |