Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae)

The first barrier, when penetrating an insect host, encountered by microorganisms like fungi is insects cuticle; fungi produce a wide variety of extracellular enzymes involved in the degradation of protein, chitin, and lipids. The main objectives were to assay the enzymatic activity of Cordyceps loc...

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Autores principales: Pelizza, Sebastián Alberto, Ferreri, Natalia, Elíades, Lorena Alejandra, Galarza, Betina, Cabello, Marta Noemí, Russo, María Leticia, Vianna, María Florencia, Scorsetti, Ana Clara, Lange, Carlos Ernesto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Química
Biological control
Entomopathogenic fungi
Enzyme production
Mortality
Pest insect
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/118815
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-118815
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Biological control
Entomopathogenic fungi
Enzyme production
Mortality
Pest insect
spellingShingle Química
Biological control
Entomopathogenic fungi
Enzyme production
Mortality
Pest insect
Pelizza, Sebastián Alberto
Ferreri, Natalia
Elíades, Lorena Alejandra
Galarza, Betina
Cabello, Marta Noemí
Russo, María Leticia
Vianna, María Florencia
Scorsetti, Ana Clara
Lange, Carlos Ernesto
Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae)
topic_facet Química
Biological control
Entomopathogenic fungi
Enzyme production
Mortality
Pest insect
description The first barrier, when penetrating an insect host, encountered by microorganisms like fungi is insects cuticle; fungi produce a wide variety of extracellular enzymes involved in the degradation of protein, chitin, and lipids. The main objectives were to assay the enzymatic activity of Cordyceps locustiphila anamorph, in solid and liquid medium at different temperatures and also to evaluate the pathogenicity against the South American locust, Schistocerca cancellata. Conidia of C. locustiphila anamorph were adjusted to 1 × 104, 1 × 106 and 1 × 108 conidia/ml and mortality was recorded for S. cancellata nymphs. The enzymatic activities were determined through a plate test and liquid medium. When assessing the pathogenicity, the fungus caused the highest mortality (84.5 ± 3.5%) at a dose of 1 × 108 conidia/ml. The proteolytic activity showed the highest values (1.56 ± 0.21) (U) at 26 °C. The highest lipolytic activity (1.13 ± 0.36) (U) was observed at 26 °C, while the highest chitinolytic activity was of 0.85 ± 0.1 (U) at 4 °C. Significant differences were observed for enzymatic production on liquid medium, the highest values were recorded for chitinolytic activity (1.63 ± 0.04) (U) and the lowest for caseinolytic activity (0.04 ± 0.001) (U). The results obtained on mortality as well as the quality and variety of enzymes produced by C. locustiphila suggest that this fungus has features that make it a good candidate to be used as a biological agent for the control of the South American locust.
format Articulo
Articulo
author Pelizza, Sebastián Alberto
Ferreri, Natalia
Elíades, Lorena Alejandra
Galarza, Betina
Cabello, Marta Noemí
Russo, María Leticia
Vianna, María Florencia
Scorsetti, Ana Clara
Lange, Carlos Ernesto
author_facet Pelizza, Sebastián Alberto
Ferreri, Natalia
Elíades, Lorena Alejandra
Galarza, Betina
Cabello, Marta Noemí
Russo, María Leticia
Vianna, María Florencia
Scorsetti, Ana Clara
Lange, Carlos Ernesto
author_sort Pelizza, Sebastián Alberto
title Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae)
title_short Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae)
title_full Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae)
title_fullStr Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae)
title_full_unstemmed Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae)
title_sort enzymatic activity and virulence of cordyceps locustiphila (hypocreales: cordycipitaceae) on the south american locust schistocerca cancellata (orthoptera: acrididae)
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/118815
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