Identification and characterization of two RNA silencing suppressors encoded by ophioviruses

Citrus psorosis virus and Mirafiori lettuce big-vein virus are two members of the genus Ophiovirus, family Ophioviridae. So far, how these viruses can interfere in the antiviral RNA silencing pathway is not known. In this study, using a local GFP silencing assay on Nicotiana benthamiana, the 24K-25K...

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Autores principales: Robles Luna, Gabriel, Reyes Martínez, Carina Andrea, Peña, Eduardo José, Ocolotobiche, Eliana Evelina, Baeza, Cecilia, Borniego, María Belén, Kormelink, Richard, García, María Laura
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
Ophiovirus
viral suppressor
RNA silencing
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/118757
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-118757
record_format dspace
spelling I19-R120-10915-1187572024-10-15T16:15:30Z http://sedici.unlp.edu.ar/handle/10915/118757 Identification and characterization of two RNA silencing suppressors encoded by ophioviruses Robles Luna, Gabriel Reyes Martínez, Carina Andrea Peña, Eduardo José Ocolotobiche, Eliana Evelina Baeza, Cecilia Borniego, María Belén Kormelink, Richard García, María Laura 2017 2021-05-12T18:53:12Z en Biología Ophiovirus viral suppressor RNA silencing Citrus psorosis virus Mirafiori lettuce big-vein virus Citrus psorosis virus and Mirafiori lettuce big-vein virus are two members of the genus Ophiovirus, family Ophioviridae. So far, how these viruses can interfere in the antiviral RNA silencing pathway is not known. In this study, using a local GFP silencing assay on Nicotiana benthamiana, the 24K-25K and the movement protein (MP) of both viruses were identified as RNA silencing suppressor proteins. Upon their co-expression with GFP in N. benthamiana 16c plants, the proteins also showed to suppress systemic RNA (GFP) silencing. The MPCPsV and 24KCPsV proteins bind long (114 nucleotides) but not short-interfering (21 nt) dsRNA, and upon transgenic expression, plants showed developmental abnormalities that coincided with an altered miRNA accumulation pattern. Furthermore, both proteins were able to suppress miRNA-induced silencing of a GFP-sensor construct and the co-expression of MPCPsV and 24KCPsV exhibited a stronger effect, suggesting they act at different stages of the RNAi pathway. Instituto de Biotecnología y Biología Molecular Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 96-105
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Ophiovirus
viral suppressor
RNA silencing
Citrus psorosis virus
Mirafiori lettuce big-vein virus
spellingShingle Biología
Ophiovirus
viral suppressor
RNA silencing
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Robles Luna, Gabriel
Reyes Martínez, Carina Andrea
Peña, Eduardo José
Ocolotobiche, Eliana Evelina
Baeza, Cecilia
Borniego, María Belén
Kormelink, Richard
García, María Laura
Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
topic_facet Biología
Ophiovirus
viral suppressor
RNA silencing
Citrus psorosis virus
Mirafiori lettuce big-vein virus
description Citrus psorosis virus and Mirafiori lettuce big-vein virus are two members of the genus Ophiovirus, family Ophioviridae. So far, how these viruses can interfere in the antiviral RNA silencing pathway is not known. In this study, using a local GFP silencing assay on Nicotiana benthamiana, the 24K-25K and the movement protein (MP) of both viruses were identified as RNA silencing suppressor proteins. Upon their co-expression with GFP in N. benthamiana 16c plants, the proteins also showed to suppress systemic RNA (GFP) silencing. The MPCPsV and 24KCPsV proteins bind long (114 nucleotides) but not short-interfering (21 nt) dsRNA, and upon transgenic expression, plants showed developmental abnormalities that coincided with an altered miRNA accumulation pattern. Furthermore, both proteins were able to suppress miRNA-induced silencing of a GFP-sensor construct and the co-expression of MPCPsV and 24KCPsV exhibited a stronger effect, suggesting they act at different stages of the RNAi pathway.
format Articulo
Articulo
author Robles Luna, Gabriel
Reyes Martínez, Carina Andrea
Peña, Eduardo José
Ocolotobiche, Eliana Evelina
Baeza, Cecilia
Borniego, María Belén
Kormelink, Richard
García, María Laura
author_facet Robles Luna, Gabriel
Reyes Martínez, Carina Andrea
Peña, Eduardo José
Ocolotobiche, Eliana Evelina
Baeza, Cecilia
Borniego, María Belén
Kormelink, Richard
García, María Laura
author_sort Robles Luna, Gabriel
title Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
title_short Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
title_full Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
title_fullStr Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
title_full_unstemmed Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
title_sort identification and characterization of two rna silencing suppressors encoded by ophioviruses
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/118757
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