MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene
At present, 53 PCR-RFLP alleles of the bovine DRB3 exon 2 were reported. In order to analyse the putative MspI restriction patterns present in the exon 2 of BoLA-DRB3 gene, all available DNA sequences were retrieved from Genbank Data Library. In this analysis, two MspI restriction sites and five res...
| Autores principales: | , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2003
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/118226 https://ri.conicet.gov.ar/11336/83778 |
| Aporte de: |
| id |
I19-R120-10915-118226 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Veterinarias BoLA-DRB3 Métodos de tipificación Msp I Polimorfismo Typing methods Polymorphism |
| spellingShingle |
Ciencias Veterinarias BoLA-DRB3 Métodos de tipificación Msp I Polimorfismo Typing methods Polymorphism Giovambattista, Guillermo Díaz, Silvina Ripoli, María Verónica Peral García, Pilar MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene |
| topic_facet |
Ciencias Veterinarias BoLA-DRB3 Métodos de tipificación Msp I Polimorfismo Typing methods Polymorphism |
| description |
At present, 53 PCR-RFLP alleles of the bovine DRB3 exon 2 were reported. In order to analyse the putative MspI restriction patterns present in the exon 2 of BoLA-DRB3 gene, all available DNA sequences were retrieved from Genbank Data Library. In this analysis, two MspI restriction sites and five restriction patterns were found. In addition, the observed restriction patterns obtained after MspI digestion of genomic DNA were compared with those predicted from the DRB3 DNA sequence analysis. The use of MspI restriction enzymes together with BstYI, HaeIII and RsaI could be useful in recognising some sequences differences in a single PCR-RFLP allele as defined by the previously used combination of enzyme, improving the robustness of this typing method designed by van Eijk et al. (1992). Despite the number of restriction patterns detected by MspI and BstYI are similar, the MspI patterns are more easily identified than those of BstYI. MspI enzyme works well in the same reaction buffer and temperature as HaeIII and RsaI, thus simplifying double digestions. Finally, the costs may be reduced. |
| format |
Articulo Articulo |
| author |
Giovambattista, Guillermo Díaz, Silvina Ripoli, María Verónica Peral García, Pilar |
| author_facet |
Giovambattista, Guillermo Díaz, Silvina Ripoli, María Verónica Peral García, Pilar |
| author_sort |
Giovambattista, Guillermo |
| title |
MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene |
| title_short |
MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene |
| title_full |
MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene |
| title_fullStr |
MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene |
| title_full_unstemmed |
MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene |
| title_sort |
mspi restriction enzyme evidence moderate polymorphism in the second exon of bola-drb3 gene |
| publishDate |
2003 |
| url |
http://sedici.unlp.edu.ar/handle/10915/118226 https://ri.conicet.gov.ar/11336/83778 |
| work_keys_str_mv |
AT giovambattistaguillermo mspirestrictionenzymeevidencemoderatepolymorphisminthesecondexonofboladrb3gene AT diazsilvina mspirestrictionenzymeevidencemoderatepolymorphisminthesecondexonofboladrb3gene AT ripolimariaveronica mspirestrictionenzymeevidencemoderatepolymorphisminthesecondexonofboladrb3gene AT peralgarciapilar mspirestrictionenzymeevidencemoderatepolymorphisminthesecondexonofboladrb3gene |
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Repositorios |
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