Identification of Campylobacters isolated from dogs by usingbiotypes and protein electrophoretic patterns

Two hundred and sixty faecal samples obtained with rectal swabs from dogs were studied for Campylobacter isolation. The samples were placed on Preston broth and incubated at 43° C for 12 h under a microaerophilicatmosphere. Then, they were seeded on Skirrow agar and incubated under the same conditio...

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Detalles Bibliográficos
Autores principales: Giacoboni, Gabriela Isabel, Puchuri, María Carmen, Castellano, María Cecilia, Echeverría, María Gabriela, Fernández, H.
Formato: Articulo
Lenguaje:Español
Publicado: 1999
Materias:
Ciencias Veterinarias
Campylobacter
Biotipificación
Electroforesis
Biotyping
Electrophoresis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/117582
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-117582
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Ciencias Veterinarias
Campylobacter
Biotipificación
Electroforesis
Biotyping
Electrophoresis
spellingShingle Ciencias Veterinarias
Campylobacter
Biotipificación
Electroforesis
Biotyping
Electrophoresis
Giacoboni, Gabriela Isabel
Puchuri, María Carmen
Castellano, María Cecilia
Echeverría, María Gabriela
Fernández, H.
Identification of Campylobacters isolated from dogs by usingbiotypes and protein electrophoretic patterns
topic_facet Ciencias Veterinarias
Campylobacter
Biotipificación
Electroforesis
Biotyping
Electrophoresis
description Two hundred and sixty faecal samples obtained with rectal swabs from dogs were studied for Campylobacter isolation. The samples were placed on Preston broth and incubated at 43° C for 12 h under a microaerophilicatmosphere. Then, they were seeded on Skirrow agar and incubated under the same conditions for 48 h. Campylobacter species were identified by conventional methods (morphological, biochemical and culturaltest), Lior's biotypes and protein profiles were examined by polyacrylamide gel electrophoresis, underreducing conditions. The prevalence of thermo-tolerant Campylobacter species was 7.3%. Fifteen out of 19 Campylobacter jejuni biotype II isolates were used in electrophoresis. Strain 1522 was employed asreference strain. Analysis of the protein profiles of the electrophoretic banding patterns presented goodcorrelation with biochemical tests. All isolates presented a protein band greater than 40-46 Kd and minordifferences in mobility of 40-50 Kd. Four strains lacked the flagellin (62-64 Kd band). The fact that proteinelectrophoresis presented heterogeneity among strains of the same species and biotypes reinforces theirimportance as discriminative methods of epidemiological value.
format Articulo
Articulo
author Giacoboni, Gabriela Isabel
Puchuri, María Carmen
Castellano, María Cecilia
Echeverría, María Gabriela
Fernández, H.
author_facet Giacoboni, Gabriela Isabel
Puchuri, María Carmen
Castellano, María Cecilia
Echeverría, María Gabriela
Fernández, H.
author_sort Giacoboni, Gabriela Isabel
title Identification of Campylobacters isolated from dogs by usingbiotypes and protein electrophoretic patterns
title_short Identification of Campylobacters isolated from dogs by usingbiotypes and protein electrophoretic patterns
title_full Identification of Campylobacters isolated from dogs by usingbiotypes and protein electrophoretic patterns
title_fullStr Identification of Campylobacters isolated from dogs by usingbiotypes and protein electrophoretic patterns
title_full_unstemmed Identification of Campylobacters isolated from dogs by usingbiotypes and protein electrophoretic patterns
title_sort identification of campylobacters isolated from dogs by usingbiotypes and protein electrophoretic patterns
publishDate 1999
url http://sedici.unlp.edu.ar/handle/10915/117582
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