Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies

Bovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope g...

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Autores principales: González, Ester Teresa, Bonzo, Estela Beatriz, Echeverría, María Gabriela, Licursi, María, Etcheverrigaray, María Elisa
Formato: Articulo
Lenguaje:Inglés
Publicado: 1999
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Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/115089
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id I19-R120-10915-115089
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Bovine Leukaemia
indirect ELISA
seroepidemiology
spellingShingle Ciencias Veterinarias
Bovine Leukaemia
indirect ELISA
seroepidemiology
González, Ester Teresa
Bonzo, Estela Beatriz
Echeverría, María Gabriela
Licursi, María
Etcheverrigaray, María Elisa
Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
topic_facet Ciencias Veterinarias
Bovine Leukaemia
indirect ELISA
seroepidemiology
description Bovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope glycoprotein and the mayor core protein p24. We describe the test procedure of an indirect ELISA (I-ELISA) using polyclonal reagents for the detection of antibodies to BLV. For comparison, the sera were simultaneously tested by agar gel immunodiffussion (AGID) test, which is currently used as diagnostic standard for BLV infection. The antigen applied does not require a high degree of purification and the data from the analysis of the negative sera showed that the establishment of a cut-off level corresponding to 3 times the standard deviation (SD) above the mean for the negative control set of sera provided acceptable specificity, reducing the risk of false positives results. A comparison of the results obtained by AGID test and I-ELISA showed that considering a total of 465 serum samples, all of the 234 samples (50%) that were positive by AGID were positive to the I-ELISA. Of 225 serum samples which yielded negative results in the AGID test, 69 (15%) were found to be positive by the I-ELISA and 156 (33%) were negative by both techniques. Few sera (2%) that were non-specific by AGID were defined as negative or positive by I-ELISA.
format Articulo
Articulo
author González, Ester Teresa
Bonzo, Estela Beatriz
Echeverría, María Gabriela
Licursi, María
Etcheverrigaray, María Elisa
author_facet González, Ester Teresa
Bonzo, Estela Beatriz
Echeverría, María Gabriela
Licursi, María
Etcheverrigaray, María Elisa
author_sort González, Ester Teresa
title Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
title_short Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
title_full Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
title_fullStr Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
title_full_unstemmed Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
title_sort enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (i-elisa) in seroepidemiological studies
publishDate 1999
url http://sedici.unlp.edu.ar/handle/10915/115089
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