Immunoprotective response induced by recombinant glycoprotein D in the BALB/c respiratory mouse model of <i>Equid alphaherpesvirus 1</i> infection

Equid alphaherpesvirus 1 (EHV-1) infection causes abortion, respiratory disease, perinatal deaths and neurological disorders in horses. The natural infection and available vaccines provide only partial and short-lived protection against reinfections. In the present study, we analyzed the ability of...

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Autores principales: Fuentealba, Nadia Analía, Sguazza, Guillermo Hernán, Zanuzzi, Carolina Natalia, Bravi, María Emilia, Scrochi, Mariela Rita, Valera, Alejandro Rafael, Corva, Santiago Gerardo, Gimeno, Eduardo Juan, Pecoraro, Marcelo Ricardo Ítalo, Galosi, Cecilia Mónica
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Veterinarias
Equid alphaherpesvirus 1
Glycoprotein D
Mouse respiratory model
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/114740
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-114740
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Equid alphaherpesvirus 1
Glycoprotein D
Mouse respiratory model
spellingShingle Ciencias Veterinarias
Equid alphaherpesvirus 1
Glycoprotein D
Mouse respiratory model
Fuentealba, Nadia Analía
Sguazza, Guillermo Hernán
Zanuzzi, Carolina Natalia
Bravi, María Emilia
Scrochi, Mariela Rita
Valera, Alejandro Rafael
Corva, Santiago Gerardo
Gimeno, Eduardo Juan
Pecoraro, Marcelo Ricardo Ítalo
Galosi, Cecilia Mónica
Immunoprotective response induced by recombinant glycoprotein D in the BALB/c respiratory mouse model of <i>Equid alphaherpesvirus 1</i> infection
topic_facet Ciencias Veterinarias
Equid alphaherpesvirus 1
Glycoprotein D
Mouse respiratory model
description Equid alphaherpesvirus 1 (EHV-1) infection causes abortion, respiratory disease, perinatal deaths and neurological disorders in horses. The natural infection and available vaccines provide only partial and short-lived protection against reinfections. In the present study, we analyzed the ability of purified baculovirus-expressed glycoprotein D (gD) administered by different routes to induce protective immunity in BALB/c mice after challenge with the EHV-1 AR8 strain. Clinical signs varied among the different groups of mice immunized by parenteral routes, and, although gD induced a specific serum IgG response, it did not prevent the virus from reaching the lungs. Intranasally immunized mice showed no clinical signs, and virus isolation from lungs, histological lesions and antigen detection by immunohistochemistry were negative. In addition, by this route, gD did not stimulate the production of serum IgG and IgA. However, a specific IgA response in the respiratory tract was confirmed in intranasally immunized mice. Thus, we conclude that the mucosal immune response could reduce the initial viral attachment and prevent the virus from reaching the lungs. Our findings provide additional data to further study new immunization strategies in the natural host.
format Articulo
Articulo
author Fuentealba, Nadia Analía
Sguazza, Guillermo Hernán
Zanuzzi, Carolina Natalia
Bravi, María Emilia
Scrochi, Mariela Rita
Valera, Alejandro Rafael
Corva, Santiago Gerardo
Gimeno, Eduardo Juan
Pecoraro, Marcelo Ricardo Ítalo
Galosi, Cecilia Mónica
author_facet Fuentealba, Nadia Analía
Sguazza, Guillermo Hernán
Zanuzzi, Carolina Natalia
Bravi, María Emilia
Scrochi, Mariela Rita
Valera, Alejandro Rafael
Corva, Santiago Gerardo
Gimeno, Eduardo Juan
Pecoraro, Marcelo Ricardo Ítalo
Galosi, Cecilia Mónica
author_sort Fuentealba, Nadia Analía
title Immunoprotective response induced by recombinant glycoprotein D in the BALB/c respiratory mouse model of <i>Equid alphaherpesvirus 1</i> infection
title_short Immunoprotective response induced by recombinant glycoprotein D in the BALB/c respiratory mouse model of <i>Equid alphaherpesvirus 1</i> infection
title_full Immunoprotective response induced by recombinant glycoprotein D in the BALB/c respiratory mouse model of <i>Equid alphaherpesvirus 1</i> infection
title_fullStr Immunoprotective response induced by recombinant glycoprotein D in the BALB/c respiratory mouse model of <i>Equid alphaherpesvirus 1</i> infection
title_full_unstemmed Immunoprotective response induced by recombinant glycoprotein D in the BALB/c respiratory mouse model of <i>Equid alphaherpesvirus 1</i> infection
title_sort immunoprotective response induced by recombinant glycoprotein d in the balb/c respiratory mouse model of <i>equid alphaherpesvirus 1</i> infection
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/114740
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