<i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo

Citrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insi...

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Detalles Bibliográficos
Autores principales: Peña, Eduardo José, Robles Luna, Gabriel, Zanek, María Cecilia, Borniego, María Belén, Reyes, Carina Andrea, Heinlein, Manfred, García, María Laura
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Biología
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Ophioviridae
Coat protein
Protein interaction
FRET
FLIM
Immunoprecipitation
Subcellular localization
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/112878
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-112878
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Ophioviridae
Coat protein
Protein interaction
FRET
FLIM
Immunoprecipitation
Subcellular localization
spellingShingle Biología
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Ophioviridae
Coat protein
Protein interaction
FRET
FLIM
Immunoprecipitation
Subcellular localization
Peña, Eduardo José
Robles Luna, Gabriel
Zanek, María Cecilia
Borniego, María Belén
Reyes, Carina Andrea
Heinlein, Manfred
García, María Laura
<i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
topic_facet Biología
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Ophioviridae
Coat protein
Protein interaction
FRET
FLIM
Immunoprecipitation
Subcellular localization
description Citrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replication cycle of these viruses, the subcellular localization of the viral coat proteins (CP) was studied. Immunoblot analysis of fractionated extracts derived from natural and experimental infected hosts indicated that the CP of CPsV occurs in the soluble cytoplasmic fraction. The cytoplasmic localization of this protein was confirmed by confocal microscopy of fluorescent protein (FP)-tagged CP following its expression in either CPsV-infected and healthy Citrus sinensis plants or in Nicotiana benthamiana plants. The same localization was observed for FP-tagged CP of MiLBVV. The CPs of CPsV and MiLBBV can undergo homologous and heterologous interactions as revealed by fluorescent lifetime imaging microscopy and co-immunoprecipitation analysis. A putative leucine zipper motif that is conserved among ophiovirus CP sequences may account for these interactions.
format Articulo
Articulo
author Peña, Eduardo José
Robles Luna, Gabriel
Zanek, María Cecilia
Borniego, María Belén
Reyes, Carina Andrea
Heinlein, Manfred
García, María Laura
author_facet Peña, Eduardo José
Robles Luna, Gabriel
Zanek, María Cecilia
Borniego, María Belén
Reyes, Carina Andrea
Heinlein, Manfred
García, María Laura
author_sort Peña, Eduardo José
title <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
title_short <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
title_full <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
title_fullStr <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
title_full_unstemmed <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
title_sort <i>citrus psorosis</i> and <i>mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/112878
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