High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells

The aim of this study was to investigate the cytotoxic and genotoxic effects of high copper (Cu) concentrations on bovine cumulus cells (CCs) cultured in vitro.We evaluated the effect of 0, 120, 240, and 360 μg/dL Cu added to in vitro maturation (IVM) medium on CC viability assessed by the trypan bl...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Anchordoquy, Juan Mateo, Anchordoquy, Juan Patricio, Nikoloff, Noelia, Pascua, Ana Malen, Furnus, Cecilia Cristina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Veterinaria
cumulus cells
MTT
Apoptosis
comet assay
copper
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/109400
https://link.springer.com/article/10.1007/s11356-017-9683-0
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-109400
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
cumulus cells
MTT
Apoptosis
comet assay
copper
spellingShingle Veterinaria
cumulus cells
MTT
Apoptosis
comet assay
copper
Anchordoquy, Juan Mateo
Anchordoquy, Juan Patricio
Nikoloff, Noelia
Pascua, Ana Malen
Furnus, Cecilia Cristina
High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells
topic_facet Veterinaria
cumulus cells
MTT
Apoptosis
comet assay
copper
description The aim of this study was to investigate the cytotoxic and genotoxic effects of high copper (Cu) concentrations on bovine cumulus cells (CCs) cultured in vitro.We evaluated the effect of 0, 120, 240, and 360 μg/dL Cu added to in vitro maturation (IVM) medium on CC viability assessed by the trypan blue (TB)–fluorescein diacetate (FDA) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays, apoptosis, and DNA damage. Differences in cell viability assessed by TB–FDAwere not significant among CC treated with 0, 120, 240, and 360 μg/dL Cu. However, mitochondrial activity assessed by MTT was lower in CC cultured with 120, 240, and 360 μg/dL Cu as compared with the control (p < 0.01). Percentages of apoptotic cells were higher when CCs were treated with 120, 240, and 360 μg/dL Cu (p < 0.05) due to higher frequencies of late apoptotic cells (p < 0.05). The frequency of live cells diminished in a dose-dependent manner when Cu was added to the culture medium. Whereas genetic damage index (GDI) increased significantly in CC cultured in the presence of 240 and 360 μg/dL Cu (p ˂ 0.05), DNA damage increased at all Cu concentrations tested (p ˂ 0.05). These results indicate that Cu induces cytotoxic and genotoxic effects in bovine CC.
format Articulo
Articulo
author Anchordoquy, Juan Mateo
Anchordoquy, Juan Patricio
Nikoloff, Noelia
Pascua, Ana Malen
Furnus, Cecilia Cristina
author_facet Anchordoquy, Juan Mateo
Anchordoquy, Juan Patricio
Nikoloff, Noelia
Pascua, Ana Malen
Furnus, Cecilia Cristina
author_sort Anchordoquy, Juan Mateo
title High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells
title_short High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells
title_full High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells
title_fullStr High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells
title_full_unstemmed High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells
title_sort high copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/109400
https://link.springer.com/article/10.1007/s11356-017-9683-0
work_keys_str_mv AT anchordoquyjuanmateo highcopperconcentrationsproducegenotoxicityandcytotoxicityinbovinecumuluscells
AT anchordoquyjuanpatricio highcopperconcentrationsproducegenotoxicityandcytotoxicityinbovinecumuluscells
AT nikoloffnoelia highcopperconcentrationsproducegenotoxicityandcytotoxicityinbovinecumuluscells
AT pascuaanamalen highcopperconcentrationsproducegenotoxicityandcytotoxicityinbovinecumuluscells
AT furnusceciliacristina highcopperconcentrationsproducegenotoxicityandcytotoxicityinbovinecumuluscells
bdutipo_str Repositorios
_version_ 1764820443827535872

Ejemplares similares