Susceptibility to peroxidation and fatty acid composition of fresh boar semen obtained from different hog farms

Objectives of this study were to determine the fatty acid composition and to analyze the sensitivity to lipid peroxidation of different boar fresh semen samples from two herds, H1 and H2. Lipid peroxidation was evaluated using chemiluminescence (cpm/mg of protein) and fatty acid profile by means of...

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Autores principales: Marmunti, Monica Edith, Gutiérrez, Ana María, Gavazza, Mariana Beatriz, Williams, Sara Inés, Palacios, Alejandro
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Veterinarias
Boar
Spermatozoa
Chemiluminescence
Polyunsaturated fatty acids
Reactive oxygen species
Cerdo
Espermatozoides
Quimioluminiscencia
Ácidos grasos polinosaturados
Especies reactivas del oxígeno
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/109312
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-109312
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Boar
Spermatozoa
Chemiluminescence
Polyunsaturated fatty acids
Reactive oxygen species
Cerdo
Espermatozoides
Quimioluminiscencia
Ácidos grasos polinosaturados
Especies reactivas del oxígeno
spellingShingle Ciencias Veterinarias
Boar
Spermatozoa
Chemiluminescence
Polyunsaturated fatty acids
Reactive oxygen species
Cerdo
Espermatozoides
Quimioluminiscencia
Ácidos grasos polinosaturados
Especies reactivas del oxígeno
Marmunti, Monica Edith
Gutiérrez, Ana María
Gavazza, Mariana Beatriz
Williams, Sara Inés
Palacios, Alejandro
Susceptibility to peroxidation and fatty acid composition of fresh boar semen obtained from different hog farms
topic_facet Ciencias Veterinarias
Boar
Spermatozoa
Chemiluminescence
Polyunsaturated fatty acids
Reactive oxygen species
Cerdo
Espermatozoides
Quimioluminiscencia
Ácidos grasos polinosaturados
Especies reactivas del oxígeno
description Objectives of this study were to determine the fatty acid composition and to analyze the sensitivity to lipid peroxidation of different boar fresh semen samples from two herds, H1 and H2. Lipid peroxidation was evaluated using chemiluminescence (cpm/mg of protein) and fatty acid profile by means of gas chromatography. The saturated fatty acid content found in the analyzed spermatozoa was approximately 43% in H1 and 33% in H2, whereas the total unsaturated fatty acid content was 47% in H1 and 59% in H2. When control and ascorbate-Fe++ dependent samples were compared, it was observed a significant increase in light emission. Consequently, significant decrease in the percentage of the polyunsaturated fatty acids was determined, being more affected: C22: 5 n6 and C22: 6 n3 in both herds, whereas C20: 4 n6 and C22: 4 n6 only in H2. The great amounts of polyunsaturated fatty acids found in H2 samples could be related to the loss of acrosomal integrity. Our results indicate that boar semen contains great amounts of polyunsaturated fatty acid in its composition, which were vulnerable to the lipid peroxidation.
format Articulo
Articulo
author Marmunti, Monica Edith
Gutiérrez, Ana María
Gavazza, Mariana Beatriz
Williams, Sara Inés
Palacios, Alejandro
author_facet Marmunti, Monica Edith
Gutiérrez, Ana María
Gavazza, Mariana Beatriz
Williams, Sara Inés
Palacios, Alejandro
author_sort Marmunti, Monica Edith
title Susceptibility to peroxidation and fatty acid composition of fresh boar semen obtained from different hog farms
title_short Susceptibility to peroxidation and fatty acid composition of fresh boar semen obtained from different hog farms
title_full Susceptibility to peroxidation and fatty acid composition of fresh boar semen obtained from different hog farms
title_fullStr Susceptibility to peroxidation and fatty acid composition of fresh boar semen obtained from different hog farms
title_full_unstemmed Susceptibility to peroxidation and fatty acid composition of fresh boar semen obtained from different hog farms
title_sort susceptibility to peroxidation and fatty acid composition of fresh boar semen obtained from different hog farms
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/109312
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