Erythrocytes modulate cell cycle progression but not the baseline frequency of sister chromatid exchanges in pig lymphocytes

The effect of co-culturing varying concentrations of pig and human red blood cells (RBCs) on the baseline frequency of sister chromatid exchanges (SCEs) and cell-cycle progression in pig plasma (PLCs) and whole blood leukocyte cultures (WBCs) was studied. No variation in SCE frequency was observed b...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Reigosa, Miguel Antonio, Soloneski, Sonia María Elsa, García, Carlos Fernando, Larramendy, Marcelo Luis
Formato: Articulo
Lenguaje:Inglés
Publicado: 1997
Materias:
Ciencias Naturales
Genética
Eritrocitos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/108731
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-108731
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Genética
Eritrocitos
spellingShingle Ciencias Naturales
Genética
Eritrocitos
Reigosa, Miguel Antonio
Soloneski, Sonia María Elsa
García, Carlos Fernando
Larramendy, Marcelo Luis
Erythrocytes modulate cell cycle progression but not the baseline frequency of sister chromatid exchanges in pig lymphocytes
topic_facet Ciencias Naturales
Genética
Eritrocitos
description The effect of co-culturing varying concentrations of pig and human red blood cells (RBCs) on the baseline frequency of sister chromatid exchanges (SCEs) and cell-cycle progression in pig plasma (PLCs) and whole blood leukocyte cultures (WBCs) was studied. No variation in SCE frequency was observed between pig control WBC and PLC. Addition of pig and human RBCs to pig PLCs did not modify the baseline frequency of SCEs. On the other hand, cell proliferation was slower in PLCs than in WBCs. The addition of pig or human RBCs to PLCs accelerated the cell-cycle progression of pig lymphocytes. When RBCs were added to PLCs the concentration and time sequence of RBC incorporation affected the cell-cycle progression of swine lymphocytes. When doses of pig or human RBCs equivalent to those present in WBCs were added immediately after PLC stimulation, the cell-cycle kinetics were similar to those of WBCs. Shorter co-incubation periods or a reduction in the dose of RBCs made cell-cycle progression intermediate between PLC and WBC values. Thus, pig and human RBCs modulated the in vitro cell-cycle progression of pig lymphocytes in a time- and dose-dependent manner, and the low baseline frequency of SCEs of pig lymphocytes is independent of the presence or absence of erythrocytes in culture.
format Articulo
Articulo
author Reigosa, Miguel Antonio
Soloneski, Sonia María Elsa
García, Carlos Fernando
Larramendy, Marcelo Luis
author_facet Reigosa, Miguel Antonio
Soloneski, Sonia María Elsa
García, Carlos Fernando
Larramendy, Marcelo Luis
author_sort Reigosa, Miguel Antonio
title Erythrocytes modulate cell cycle progression but not the baseline frequency of sister chromatid exchanges in pig lymphocytes
title_short Erythrocytes modulate cell cycle progression but not the baseline frequency of sister chromatid exchanges in pig lymphocytes
title_full Erythrocytes modulate cell cycle progression but not the baseline frequency of sister chromatid exchanges in pig lymphocytes
title_fullStr Erythrocytes modulate cell cycle progression but not the baseline frequency of sister chromatid exchanges in pig lymphocytes
title_full_unstemmed Erythrocytes modulate cell cycle progression but not the baseline frequency of sister chromatid exchanges in pig lymphocytes
title_sort erythrocytes modulate cell cycle progression but not the baseline frequency of sister chromatid exchanges in pig lymphocytes
publishDate 1997
url http://sedici.unlp.edu.ar/handle/10915/108731
work_keys_str_mv AT reigosamiguelantonio erythrocytesmodulatecellcycleprogressionbutnotthebaselinefrequencyofsisterchromatidexchangesinpiglymphocytes
AT soloneskisoniamariaelsa erythrocytesmodulatecellcycleprogressionbutnotthebaselinefrequencyofsisterchromatidexchangesinpiglymphocytes
AT garciacarlosfernando erythrocytesmodulatecellcycleprogressionbutnotthebaselinefrequencyofsisterchromatidexchangesinpiglymphocytes
AT larramendymarceloluis erythrocytesmodulatecellcycleprogressionbutnotthebaselinefrequencyofsisterchromatidexchangesinpiglymphocytes
bdutipo_str Repositorios
_version_ 1764820443961753601

Ejemplares similares