Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin

Protopectinases (PPases) constitute a heterogeneous group of extracellular enzymes able to release soluble pectin from insoluble protopectin in plant tissues. Geotrichum klebahnii (ATCC 42397) produces PPase-SE with endopolygalacturonase activity. PPase-SE has been used for pectin extraction and mac...

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Autores principales: Rojas, Natalia Lorena, Cavalitto, Sebastián Fernando, Mignone, Carlos Fernando, Hours, Roque Alberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2008
Materias:
Química
Carbon balance
Enzymatic pectin extraction
Microbial pectin utilization
Pectinases
Synthetic medium
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/108601
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-108601
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Carbon balance
Enzymatic pectin extraction
Microbial pectin utilization
Pectinases
Synthetic medium
spellingShingle Química
Carbon balance
Enzymatic pectin extraction
Microbial pectin utilization
Pectinases
Synthetic medium
Rojas, Natalia Lorena
Cavalitto, Sebastián Fernando
Mignone, Carlos Fernando
Hours, Roque Alberto
Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
topic_facet Química
Carbon balance
Enzymatic pectin extraction
Microbial pectin utilization
Pectinases
Synthetic medium
description Protopectinases (PPases) constitute a heterogeneous group of extracellular enzymes able to release soluble pectin from insoluble protopectin in plant tissues. Geotrichum klebahnii (ATCC 42397) produces PPase-SE with endopolygalacturonase activity. PPase-SE has been used for pectin extraction and maceration of plant tissues. Here, the capacity of G. klebahnii to use different pectins as carbon and energy sources (CES) was studied, in addition to PPase-SE capacity to release pectin from lemon peel. The strain was unable to use pectin from different origins as CES. When G. klebahnii was cultivated with mixtures of different amounts of glucose and citrus pectin as CES, the biomass obtained was proportional to the initial concentration of glucose, which was completely consumed. In addition, it produced PPase-SE in a glucose-containing medium. A culture was used for the extraction of pectin from lemon peels. Pectin was enzymatically extracted simultaneously with tissue maceration, yielding 3.7 g of (dry) pectin per 100 g of (wet) lemon peel. Extracted pectin was not metabolized by the strain. It was concluded that G. klebahnii uses PPase-SE to macerate, invade and colonize plant tissues, thus releasing soluble sugars to be used as CES without metabolizing solubilized pectin.
format Articulo
Articulo
author Rojas, Natalia Lorena
Cavalitto, Sebastián Fernando
Mignone, Carlos Fernando
Hours, Roque Alberto
author_facet Rojas, Natalia Lorena
Cavalitto, Sebastián Fernando
Mignone, Carlos Fernando
Hours, Roque Alberto
author_sort Rojas, Natalia Lorena
title Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
title_short Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
title_full Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
title_fullStr Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
title_full_unstemmed Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
title_sort role of ppase-se in geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
publishDate 2008
url http://sedici.unlp.edu.ar/handle/10915/108601
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