Behavior of Araujiain, a new cysteine phytoprotease, in organic media with low water content

In this paper we studied the effect of different organic solvents (1-octanol, trichloroethylene, ethanol, ethyl acetate, tetrahydrofuran, cyclohexane, propanone, acetonitrile, dichloromethane, chlorobenzene, N,Ndimethylformamide, acetophenone, diethyl ether, methanol, ethylene glycol and toluene) wi...

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Detalles Bibliográficos
Autores principales:	Quiroga, Evelina, Priolo de Lufrano, Nora Silvia, Marchese, José, Barberis, Sonia
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2006
Materias:	Química Araujia hortorum Fourn Organic solvents Plant protease Substrate preferences Thermostability
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/108593
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-108593
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Química Araujia hortorum Fourn Organic solvents Plant protease Substrate preferences Thermostability
spellingShingle	Química Araujia hortorum Fourn Organic solvents Plant protease Substrate preferences Thermostability Quiroga, Evelina Priolo de Lufrano, Nora Silvia Marchese, José Barberis, Sonia Behavior of Araujiain, a new cysteine phytoprotease, in organic media with low water content
topic_facet	Química Araujia hortorum Fourn Organic solvents Plant protease Substrate preferences Thermostability
description	In this paper we studied the effect of different organic solvents (1-octanol, trichloroethylene, ethanol, ethyl acetate, tetrahydrofuran, cyclohexane, propanone, acetonitrile, dichloromethane, chlorobenzene, N,Ndimethylformamide, acetophenone, diethyl ether, methanol, ethylene glycol and toluene) with low and constant water content on substrate preferences, thermostability and stability (caseinolytic activity retention after 4 h) of proteases of Araujia hortorum Fourn. (Asclepiadaceae). The stability of araujiain was high in N,N-dimethylformamide and ethanol at 40ºC, but decreased at higher temperature. Araujiain substrates preferences in buffer Tris-HCl (pH 8), ethylene glycol and N,N-dimethylformamide exhibited different patterns, but the enzyme showed a high preference by glutamine derivative in all cases. According to FTIR spectroscopy studies, araujiain changed its secondary structure and as a consequence, it also changed its substrate preferences. This enzyme showed lower α-helical character and greater β-sheet folding in buffer than in organic media. A larger amount of antiparallel β-sheet residues indicates the formation of tighter intermolecular hydrogen bonds and enzymatic aggregates. These facts could explain the higher esterolytic activities, the greater stability and good hydrolytic potential of araujiain in some organic media such as N,N-dimethylformamide.
format	Articulo Articulo
author	Quiroga, Evelina Priolo de Lufrano, Nora Silvia Marchese, José Barberis, Sonia
author_facet	Quiroga, Evelina Priolo de Lufrano, Nora Silvia Marchese, José Barberis, Sonia
author_sort	Quiroga, Evelina
title	Behavior of Araujiain, a new cysteine phytoprotease, in organic media with low water content
title_short	Behavior of Araujiain, a new cysteine phytoprotease, in organic media with low water content
title_full	Behavior of Araujiain, a new cysteine phytoprotease, in organic media with low water content
title_fullStr	Behavior of Araujiain, a new cysteine phytoprotease, in organic media with low water content
title_full_unstemmed	Behavior of Araujiain, a new cysteine phytoprotease, in organic media with low water content
title_sort	behavior of araujiain, a new cysteine phytoprotease, in organic media with low water content
publishDate	2006
url	http://sedici.unlp.edu.ar/handle/10915/108593
work_keys_str_mv	AT quirogaevelina behaviorofaraujiainanewcysteinephytoproteaseinorganicmediawithlowwatercontent AT priolodelufranonorasilvia behaviorofaraujiainanewcysteinephytoproteaseinorganicmediawithlowwatercontent AT marchesejose behaviorofaraujiainanewcysteinephytoproteaseinorganicmediawithlowwatercontent AT barberissonia behaviorofaraujiainanewcysteinephytoproteaseinorganicmediawithlowwatercontent
bdutipo_str	Repositorios
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Behavior of Araujiain, a new cysteine phytoprotease, in organic media with low water content

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