An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis

A highly efficient somatic embryogenesis system and subsequent plant regeneration of chinaberry (Melia azedarach L.) was developed. Plants were regenerated from indirect somatic embryogenesis induction. Novel features of this improved protocol, include: a) Embryogenic callus induction with no additi...

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Autores principales:	Sharry, Sandra Elizabeth, Cabrera Ponce, José Luis, Herrera Estrella, Luis, Rangel Cano, Rosa María, Lede, Silvia, Abedini, Walter
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2006
Materias:	Ciencias Agrarias Melia azedarach L. Somatic embryogenesis system
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/108589
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-108589
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Agrarias Melia azedarach L. Somatic embryogenesis system
spellingShingle	Ciencias Agrarias Melia azedarach L. Somatic embryogenesis system Sharry, Sandra Elizabeth Cabrera Ponce, José Luis Herrera Estrella, Luis Rangel Cano, Rosa María Lede, Silvia Abedini, Walter An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
topic_facet	Ciencias Agrarias Melia azedarach L. Somatic embryogenesis system
description	A highly efficient somatic embryogenesis system and subsequent plant regeneration of chinaberry (Melia azedarach L.) was developed. Plants were regenerated from indirect somatic embryogenesis induction. Novel features of this improved protocol, include: a) Embryogenic callus induction with no addition of 2, 4-D in the culture media; b) Somatic embryos differentiation was achieved by using high concentration of cytokinins (BAP 10 mg/L) and adenine; c) 100% conversion of somatic embryos to plants was practically obtained and 100% of plants survived under greenhouse conditions; d) Addition of putrescine improved somatic embryos germination. The amount of somatic embryos produced by the pathway of indirect somatic embryogenesis was 447 per gram of fresh weight callus. Regenerated plants were phenotypically normal. The developed protocol established the potential to produce plantlets from cotyledon explants through somatic embryogenesis. It also presents itself as a highly efficient method for mass clonal propagation and conservation of Melia azedarach.
format	Articulo Articulo
author	Sharry, Sandra Elizabeth Cabrera Ponce, José Luis Herrera Estrella, Luis Rangel Cano, Rosa María Lede, Silvia Abedini, Walter
author_facet	Sharry, Sandra Elizabeth Cabrera Ponce, José Luis Herrera Estrella, Luis Rangel Cano, Rosa María Lede, Silvia Abedini, Walter
author_sort	Sharry, Sandra Elizabeth
title	An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
title_short	An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
title_full	An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
title_fullStr	An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
title_full_unstemmed	An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
title_sort	alternative pathway for plant in vitro regeneration of chinaberry -tree melia azedarach l. derived from the induction of somatic embryogenesis
publishDate	2006
url	http://sedici.unlp.edu.ar/handle/10915/108589
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bdutipo_str	Repositorios
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An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis

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