Investigation of apoptosis in cultured cells infected with equine herpesvirus 1

Many viruses alter different stages of apoptosis of infected cells as a strategy for successful infection. Few studies have addressed mechanisms of equine herpesvirus 1 (EHV-1) strain-induced cell death. We investigated the effect of an abortigenic strain (AR8 strain) on heterologous Madin–Darby bov...

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Autores principales:	Scrochi, Mariela Rita, Zanuzzi, Carolina Natalia, Fuentealba, Nadia Analía, Nishida, Fabián, Bravi, María Emilia, Pacheco, María Emilia, Sguazza, Guillermo Hernán, Gimeno, Eduardo Juan, Portiansky, Enrique Leo, Muglia, Cecilia Isabel, Galosi, Cecilia Mónica, Barbeito, Claudio Gustavo
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2017
Materias:	Veterinaria Ciencias Exactas Apoptosis equine dermis cells equine herpes virus 1 Madin–Darby bovine kidney cells
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/108503 https://www.tandfonline.com/doi/full/10.1080/10520295.2017.1359749
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-108503
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Veterinaria Ciencias Exactas Apoptosis equine dermis cells equine herpes virus 1 Madin–Darby bovine kidney cells
spellingShingle	Veterinaria Ciencias Exactas Apoptosis equine dermis cells equine herpes virus 1 Madin–Darby bovine kidney cells Scrochi, Mariela Rita Zanuzzi, Carolina Natalia Fuentealba, Nadia Analía Nishida, Fabián Bravi, María Emilia Pacheco, María Emilia Sguazza, Guillermo Hernán Gimeno, Eduardo Juan Portiansky, Enrique Leo Muglia, Cecilia Isabel Galosi, Cecilia Mónica Barbeito, Claudio Gustavo Investigation of apoptosis in cultured cells infected with equine herpesvirus 1
topic_facet	Veterinaria Ciencias Exactas Apoptosis equine dermis cells equine herpes virus 1 Madin–Darby bovine kidney cells
description	Many viruses alter different stages of apoptosis of infected cells as a strategy for successful infection. Few studies have addressed mechanisms of equine herpesvirus 1 (EHV-1) strain-induced cell death. We investigated the effect of an abortigenic strain (AR8 strain) on heterologous Madin–Darby bovine kidney cells and homologous equine dermis (ED) cells cell lines. We compared morphologic and biochemical features of early and late apoptosis at different postinfection times. We investigated translocation of phosphatidylserine to the cell surface, nuclear fragmentation and changes in the cytoskeleton using flow cytometry and annexin V/propidium iodide staining, DNA laddering, terminal deoxynucleotidyl transferase UTP nick-end labeling assay and immunofluorescence staining of cytokeratin 18 cleavage. AR8 EVH-1 strain interfered with apoptosis in both cell lines, particularly during the middle stage of the replication cycle; this was more evident in ED cells. Although this antiapoptotic effect has been reported for other alpha herpesviruses, our findings may help elucidate how EHV-1 improves its infectivity during its cycle.
format	Articulo Articulo
author	Scrochi, Mariela Rita Zanuzzi, Carolina Natalia Fuentealba, Nadia Analía Nishida, Fabián Bravi, María Emilia Pacheco, María Emilia Sguazza, Guillermo Hernán Gimeno, Eduardo Juan Portiansky, Enrique Leo Muglia, Cecilia Isabel Galosi, Cecilia Mónica Barbeito, Claudio Gustavo
author_facet	Scrochi, Mariela Rita Zanuzzi, Carolina Natalia Fuentealba, Nadia Analía Nishida, Fabián Bravi, María Emilia Pacheco, María Emilia Sguazza, Guillermo Hernán Gimeno, Eduardo Juan Portiansky, Enrique Leo Muglia, Cecilia Isabel Galosi, Cecilia Mónica Barbeito, Claudio Gustavo
author_sort	Scrochi, Mariela Rita
title	Investigation of apoptosis in cultured cells infected with equine herpesvirus 1
title_short	Investigation of apoptosis in cultured cells infected with equine herpesvirus 1
title_full	Investigation of apoptosis in cultured cells infected with equine herpesvirus 1
title_fullStr	Investigation of apoptosis in cultured cells infected with equine herpesvirus 1
title_full_unstemmed	Investigation of apoptosis in cultured cells infected with equine herpesvirus 1
title_sort	investigation of apoptosis in cultured cells infected with equine herpesvirus 1
publishDate	2017
url	http://sedici.unlp.edu.ar/handle/10915/108503 https://www.tandfonline.com/doi/full/10.1080/10520295.2017.1359749
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Investigation of apoptosis in cultured cells infected with equine herpesvirus 1

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