An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-<i>Neospora caninum</i> antibodies in cattle
We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero- American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (...
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| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/108498 |
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I19-R120-10915-108498 |
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dspace |
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Universidad Nacional de La Plata |
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I-19 |
| repository_str |
R-120 |
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SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Veterinarias Bovine neosporosis Serological assay trial Ibero-America |
| spellingShingle |
Ciencias Veterinarias Bovine neosporosis Serological assay trial Ibero-America Campero, Lucía María Moreno Gonzalo, Javier Venturini, María Cecilia Moré, Gastón Andrés Dellarupe, Andrea Rambeaud, Magdalena Echaide, Ignacio E. Valentini, Beatriz Campero, Carlos Manuel Moore, Dadín Prando Cano, Dora B. Fort, Marcelo Mota, Rinaldo A. Serrano Martínez, Marcos E. Cruz Vázquez, Carlos Ortega Mora, Luis M. Álvarez García, Gema An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-<i>Neospora caninum</i> antibodies in cattle |
| topic_facet |
Ciencias Veterinarias Bovine neosporosis Serological assay trial Ibero-America |
| description |
We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero- American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (n = 1), Mexico (n = 1), and Spain (n = 1). A blind panel of well-characterized cattle sera (n = 143) and sera representative of the target population (n = 351) was tested by seven in-house indirect fluorescent antibody tests (IFATs 1–7) and three enzyme-linked immunosorbent assays (ELISAs 1–3; two in-house and one commercial). Diagnostic performance of the serological tests was calculated and compared according to the following criteria: (1) the BPre-test information,^ which uses previous epidemiological and serological data; (2) the BMajority of tests,^ which classifies a serumas positive or negative according to the results obtained by most tests evaluated. Unexpectedly, six tests showed either sensitivity (Se) or specificity (Sp) values lower than 90%. In contrast, the best tests in terms of Se, Sp, and area under the ROC curve (AUC) values were IFAT 1 and optimized ELISA 1 and ELISA 2. We evaluated a high number of IFATs, which are the most widely used tests in Ibero-America. The significant discordances observed among the tests regardless of the criteria employed hinder control programs and urge the use of a common test or with similar performances to either the optimized IFAT 1 and ELISAs 1 and 2. |
| format |
Articulo Articulo |
| author |
Campero, Lucía María Moreno Gonzalo, Javier Venturini, María Cecilia Moré, Gastón Andrés Dellarupe, Andrea Rambeaud, Magdalena Echaide, Ignacio E. Valentini, Beatriz Campero, Carlos Manuel Moore, Dadín Prando Cano, Dora B. Fort, Marcelo Mota, Rinaldo A. Serrano Martínez, Marcos E. Cruz Vázquez, Carlos Ortega Mora, Luis M. Álvarez García, Gema |
| author_facet |
Campero, Lucía María Moreno Gonzalo, Javier Venturini, María Cecilia Moré, Gastón Andrés Dellarupe, Andrea Rambeaud, Magdalena Echaide, Ignacio E. Valentini, Beatriz Campero, Carlos Manuel Moore, Dadín Prando Cano, Dora B. Fort, Marcelo Mota, Rinaldo A. Serrano Martínez, Marcos E. Cruz Vázquez, Carlos Ortega Mora, Luis M. Álvarez García, Gema |
| author_sort |
Campero, Lucía María |
| title |
An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-<i>Neospora caninum</i> antibodies in cattle |
| title_short |
An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-<i>Neospora caninum</i> antibodies in cattle |
| title_full |
An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-<i>Neospora caninum</i> antibodies in cattle |
| title_fullStr |
An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-<i>Neospora caninum</i> antibodies in cattle |
| title_full_unstemmed |
An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-<i>Neospora caninum</i> antibodies in cattle |
| title_sort |
ibero-american inter-laboratory trial to evaluate serological tests for the detection of anti-<i>neospora caninum</i> antibodies in cattle |
| publishDate |
2017 |
| url |
http://sedici.unlp.edu.ar/handle/10915/108498 |
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