Analyses of apoptosis and DNA damage in bovine cumulus cells after <i>in vitro</i> maturation with different copper concentration : Consequences on early embryo development

The aim of this study was to investigate the influence of copper (Cu) during in vitro maturation (IVM) on apoptosis and DNA integrity of cumulus cells (CC); and oocyte viability. Also, the role of CC in the transport of Cu during IVM was evaluated on oocyte developmental capacity. Damage of DNA was...

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Autores principales: Rosa, Diana Esther, Anchordoquy, Juan Mateo, Anchordoquy, Juan Patricio, Sirini, Matías Ángel, Testa, Juan Alberto, Mattioli, Guillermo Alberto, Furnus, Cecilia Cristina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Veterinarias
Apoptosis
DNA damage
Early development
Minerals
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/108334
Aporte de:
Aportado por : SEDICI (UNLP) de Universidad Nacional de La Plata .
id I19-R120-10915-108334
record_format dspace
spelling I19-R120-10915-1083342020-11-07T04:07:02Z http://sedici.unlp.edu.ar/handle/10915/108334 issn:1469-8730 Analyses of apoptosis and DNA damage in bovine cumulus cells after <i>in vitro</i> maturation with different copper concentration : Consequences on early embryo development Rosa, Diana Esther Anchordoquy, Juan Mateo Anchordoquy, Juan Patricio Sirini, Matías Ángel Testa, Juan Alberto Mattioli, Guillermo Alberto Furnus, Cecilia Cristina 2016 2020-11-04T21:17:00Z en Ciencias Veterinarias Apoptosis DNA damage Early development Minerals The aim of this study was to investigate the influence of copper (Cu) during in vitro maturation (IVM) on apoptosis and DNA integrity of cumulus cells (CC); and oocyte viability. Also, the role of CC in the transport of Cu during IVM was evaluated on oocyte developmental capacity. Damage of DNA was higher in CC matured without Cu (0 µg/dl Cu, P < 0.01) with respect to cells treated with Cu for cumulus–oocyte complexes (COCs) exposed to 0, 20, 40, or 60 µg/dl Cu). The percentage of apoptotic cells was higher in CC matured without Cu than in CC matured with Cu. Cumulus expansion and viability of CC did not show differences in COC treated with 0, 20, 40, or 60 µg/dl Cu during IVM. After in vitro fertilization (IVF), cleavage rates were higher in COC and DO + CC (denuded oocytes + CC) with or without Cu than in DO. Independently of CC presence (COC, DO + CC or DO) the blastocyst rates were higher when 60 µg/dl Cu was added to IVM medium compared to medium alone. These results indicate that Cu supplementation to IVM medium: (i) decreased DNA damage and apoptosis in CC; (ii) did not modify oocyte viability and cumulus expansion; and (iii) improved subsequent embryo development up to blastocyst stage regardless of CC presence during IVM. Instituto de Genética Veterinaria Facultad de Ciencias Veterinarias Facultad de Ciencias Médicas Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 869-879
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Apoptosis
DNA damage
Early development
Minerals
spellingShingle Ciencias Veterinarias
Apoptosis
DNA damage
Early development
Minerals
Rosa, Diana Esther
Anchordoquy, Juan Mateo
Anchordoquy, Juan Patricio
Sirini, Matías Ángel
Testa, Juan Alberto
Mattioli, Guillermo Alberto
Furnus, Cecilia Cristina
Analyses of apoptosis and DNA damage in bovine cumulus cells after <i>in vitro</i> maturation with different copper concentration : Consequences on early embryo development
topic_facet Ciencias Veterinarias
Apoptosis
DNA damage
Early development
Minerals
description The aim of this study was to investigate the influence of copper (Cu) during in vitro maturation (IVM) on apoptosis and DNA integrity of cumulus cells (CC); and oocyte viability. Also, the role of CC in the transport of Cu during IVM was evaluated on oocyte developmental capacity. Damage of DNA was higher in CC matured without Cu (0 µg/dl Cu, P < 0.01) with respect to cells treated with Cu for cumulus–oocyte complexes (COCs) exposed to 0, 20, 40, or 60 µg/dl Cu). The percentage of apoptotic cells was higher in CC matured without Cu than in CC matured with Cu. Cumulus expansion and viability of CC did not show differences in COC treated with 0, 20, 40, or 60 µg/dl Cu during IVM. After in vitro fertilization (IVF), cleavage rates were higher in COC and DO + CC (denuded oocytes + CC) with or without Cu than in DO. Independently of CC presence (COC, DO + CC or DO) the blastocyst rates were higher when 60 µg/dl Cu was added to IVM medium compared to medium alone. These results indicate that Cu supplementation to IVM medium: (i) decreased DNA damage and apoptosis in CC; (ii) did not modify oocyte viability and cumulus expansion; and (iii) improved subsequent embryo development up to blastocyst stage regardless of CC presence during IVM.
format Articulo
Articulo
author Rosa, Diana Esther
Anchordoquy, Juan Mateo
Anchordoquy, Juan Patricio
Sirini, Matías Ángel
Testa, Juan Alberto
Mattioli, Guillermo Alberto
Furnus, Cecilia Cristina
author_facet Rosa, Diana Esther
Anchordoquy, Juan Mateo
Anchordoquy, Juan Patricio
Sirini, Matías Ángel
Testa, Juan Alberto
Mattioli, Guillermo Alberto
Furnus, Cecilia Cristina
author_sort Rosa, Diana Esther
title Analyses of apoptosis and DNA damage in bovine cumulus cells after <i>in vitro</i> maturation with different copper concentration : Consequences on early embryo development
title_short Analyses of apoptosis and DNA damage in bovine cumulus cells after <i>in vitro</i> maturation with different copper concentration : Consequences on early embryo development
title_full Analyses of apoptosis and DNA damage in bovine cumulus cells after <i>in vitro</i> maturation with different copper concentration : Consequences on early embryo development
title_fullStr Analyses of apoptosis and DNA damage in bovine cumulus cells after <i>in vitro</i> maturation with different copper concentration : Consequences on early embryo development
title_full_unstemmed Analyses of apoptosis and DNA damage in bovine cumulus cells after <i>in vitro</i> maturation with different copper concentration : Consequences on early embryo development
title_sort analyses of apoptosis and dna damage in bovine cumulus cells after <i>in vitro</i> maturation with different copper concentration : consequences on early embryo development
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/108334
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