<i>Burkholderia puraquae</i> sp. nov., a novel species of the <i>Burkholderia cepacia</i> complex isolated from hospital settings and agricultural soils

Bacteria from the <i>Burkholderia cepacia</i> complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). These opportunistic pathogens are also widely distributed in natural and man-made environments. After a 12-year epidemiological surveillance involvi...

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Detalles Bibliográficos
Autores principales: Martina, Pablo Francisco, Leguizamón, Mariana, Prieto, Claudia Inés, Sousa, Silvia A., Montanaro, Patricia, Draghi, Walter Omar, Stämmler, Maren, Bettiol, Marisa, Carvalho, Carla C. C. R. de, Palau, Juliana, Fígoli, Cecilia Beatriz, Alvarez, Florencia, Benetti, Silvina, Lejona, Sergio, Vescina, Cecilia, Ferreras, Julián, Lasch, Peter, Lagares, Antonio, Zorreguieta, Ángeles, Leitão, Jorge H., Yantorno, Osvaldo Miguel, Bosch, María Alejandra
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Exactas
Biología
Burkholderia genus
Burkholderia cepacia complex
Burkholderia puraquae sp. nov.
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/108302
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002293
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
Descripción
Sumario:Bacteria from the <i>Burkholderia cepacia</i> complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). These opportunistic pathogens are also widely distributed in natural and man-made environments. After a 12-year epidemiological surveillance involving Bcc bacteria from respiratory secretions of Argentinean patients with CF and from hospital settings, we found six isolates of the Bcc with a concatenated species-specific allele sequence that differed by more than 3% from those of the Bcc with validly published names. According to the multilocus sequence analysis (MLSA), these isolates clustered with the agricultural soil strain, <i>Burkholderia</i> sp. PBP 78, which was already deposited in the PubMLST database. The isolates were examined using a polyphasic approach, which included 16S rRNA, <i>recA</i>, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), DNA base composition, average nucleotide identities (ANIs), fatty acid profiles, and biochemical characterizations. The results of the present study demonstrate that the seven isolates represent a single novel species within the Bcc, for which the name <i>Burkholderia puraquae</i> sp. nov. is proposed. <i>Burkholderia puraquae</i> sp. nov. CAMPA 1040<sup>T</sup> (=LMG 29660<sup>T</sup>=DSM 103137<sup>T</sup>) was designated the type strain of the novel species, which can be differentiated from other species of the Bcc mainly from <i>recA</i> gene sequence analysis, MLSA, ANIb, MALDI-TOF MS analysis, and some biochemical tests, including the ability to grow at 42 ºC, aesculin hydrolysis, and lysine decarboxylase and β-galactosidase activities.

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