<i>Burkholderia puraquae</i> sp. nov., a novel species of the <i>Burkholderia cepacia</i> complex isolated from hospital settings and agricultural soils
Bacteria from the <i>Burkholderia cepacia</i> complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). These opportunistic pathogens are also widely distributed in natural and man-made environments. After a 12-year epidemiological surveillance involvi...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/108302 https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002293 |
| Aporte de: |
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I19-R120-10915-108302 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Biología Burkholderia genus Burkholderia cepacia complex Burkholderia puraquae sp. nov. |
| spellingShingle |
Ciencias Exactas Biología Burkholderia genus Burkholderia cepacia complex Burkholderia puraquae sp. nov. Martina, Pablo Francisco Leguizamón, Mariana Prieto, Claudia Inés Sousa, Silvia A. Montanaro, Patricia Draghi, Walter Omar Stämmler, Maren Bettiol, Marisa Carvalho, Carla C. C. R. de Palau, Juliana Fígoli, Cecilia Beatriz Alvarez, Florencia Benetti, Silvina Lejona, Sergio Vescina, Cecilia Ferreras, Julián Lasch, Peter Lagares, Antonio Zorreguieta, Ángeles Leitão, Jorge H. Yantorno, Osvaldo Miguel Bosch, María Alejandra <i>Burkholderia puraquae</i> sp. nov., a novel species of the <i>Burkholderia cepacia</i> complex isolated from hospital settings and agricultural soils |
| topic_facet |
Ciencias Exactas Biología Burkholderia genus Burkholderia cepacia complex Burkholderia puraquae sp. nov. |
| description |
Bacteria from the <i>Burkholderia cepacia</i> complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). These opportunistic pathogens are also widely distributed in natural and man-made environments. After a 12-year epidemiological surveillance involving Bcc bacteria from respiratory secretions of Argentinean patients with CF and from hospital settings, we found six isolates of the Bcc with a concatenated species-specific allele sequence that differed by more than 3% from those of the Bcc with validly published names. According to the multilocus sequence analysis (MLSA), these isolates clustered with the agricultural soil strain, <i>Burkholderia</i> sp. PBP 78, which was already deposited in the PubMLST database. The isolates were examined using a polyphasic approach, which included 16S rRNA, <i>recA</i>, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), DNA base composition, average nucleotide identities (ANIs), fatty acid profiles, and biochemical characterizations. The results of the present study demonstrate that the seven isolates represent a single novel species within the Bcc, for which the name <i>Burkholderia puraquae</i> sp. nov. is proposed. <i>Burkholderia puraquae</i> sp. nov. CAMPA 1040<sup>T</sup> (=LMG 29660<sup>T</sup>=DSM 103137<sup>T</sup>) was designated the type strain of the novel species, which can be differentiated from other species of the Bcc mainly from <i>recA</i> gene sequence analysis, MLSA, ANIb, MALDI-TOF MS analysis, and some biochemical tests, including the ability to grow at 42 ºC, aesculin hydrolysis, and lysine decarboxylase and β-galactosidase activities. |
| format |
Articulo Articulo |
| author |
Martina, Pablo Francisco Leguizamón, Mariana Prieto, Claudia Inés Sousa, Silvia A. Montanaro, Patricia Draghi, Walter Omar Stämmler, Maren Bettiol, Marisa Carvalho, Carla C. C. R. de Palau, Juliana Fígoli, Cecilia Beatriz Alvarez, Florencia Benetti, Silvina Lejona, Sergio Vescina, Cecilia Ferreras, Julián Lasch, Peter Lagares, Antonio Zorreguieta, Ángeles Leitão, Jorge H. Yantorno, Osvaldo Miguel Bosch, María Alejandra |
| author_facet |
Martina, Pablo Francisco Leguizamón, Mariana Prieto, Claudia Inés Sousa, Silvia A. Montanaro, Patricia Draghi, Walter Omar Stämmler, Maren Bettiol, Marisa Carvalho, Carla C. C. R. de Palau, Juliana Fígoli, Cecilia Beatriz Alvarez, Florencia Benetti, Silvina Lejona, Sergio Vescina, Cecilia Ferreras, Julián Lasch, Peter Lagares, Antonio Zorreguieta, Ángeles Leitão, Jorge H. Yantorno, Osvaldo Miguel Bosch, María Alejandra |
| author_sort |
Martina, Pablo Francisco |
| title |
<i>Burkholderia puraquae</i> sp. nov., a novel species of the <i>Burkholderia cepacia</i> complex isolated from hospital settings and agricultural soils |
| title_short |
<i>Burkholderia puraquae</i> sp. nov., a novel species of the <i>Burkholderia cepacia</i> complex isolated from hospital settings and agricultural soils |
| title_full |
<i>Burkholderia puraquae</i> sp. nov., a novel species of the <i>Burkholderia cepacia</i> complex isolated from hospital settings and agricultural soils |
| title_fullStr |
<i>Burkholderia puraquae</i> sp. nov., a novel species of the <i>Burkholderia cepacia</i> complex isolated from hospital settings and agricultural soils |
| title_full_unstemmed |
<i>Burkholderia puraquae</i> sp. nov., a novel species of the <i>Burkholderia cepacia</i> complex isolated from hospital settings and agricultural soils |
| title_sort |
<i>burkholderia puraquae</i> sp. nov., a novel species of the <i>burkholderia cepacia</i> complex isolated from hospital settings and agricultural soils |
| publishDate |
2018 |
| url |
http://sedici.unlp.edu.ar/handle/10915/108302 https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002293 |
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