An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria

Flavin-binding fuorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efcacy of eleven LOV-based FPs. In particular, we determined single...

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Autores principales: Endres, Stephan, Wingen, Marcus, Torra, Joaquim, Ruiz González, Rubén, Polen, Tino, Bosio, Gabriela Natalia, Bitzenhofer, Nora Lisa, Hilgers, Fabienne, Gensch, Thomas, Nonell, Santi, Jaeger, Karl-Erich, Drepper, Thomas
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Química
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/108133
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6177443&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-108133
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
spellingShingle Química
Endres, Stephan
Wingen, Marcus
Torra, Joaquim
Ruiz González, Rubén
Polen, Tino
Bosio, Gabriela Natalia
Bitzenhofer, Nora Lisa
Hilgers, Fabienne
Gensch, Thomas
Nonell, Santi
Jaeger, Karl-Erich
Drepper, Thomas
An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
topic_facet Química
description Flavin-binding fuorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efcacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested favoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable diferences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specifc ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications.
format Articulo
Articulo
author Endres, Stephan
Wingen, Marcus
Torra, Joaquim
Ruiz González, Rubén
Polen, Tino
Bosio, Gabriela Natalia
Bitzenhofer, Nora Lisa
Hilgers, Fabienne
Gensch, Thomas
Nonell, Santi
Jaeger, Karl-Erich
Drepper, Thomas
author_facet Endres, Stephan
Wingen, Marcus
Torra, Joaquim
Ruiz González, Rubén
Polen, Tino
Bosio, Gabriela Natalia
Bitzenhofer, Nora Lisa
Hilgers, Fabienne
Gensch, Thomas
Nonell, Santi
Jaeger, Karl-Erich
Drepper, Thomas
author_sort Endres, Stephan
title An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_short An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_full An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_fullStr An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_full_unstemmed An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_sort optogenetic toolbox of lov-based photosensitizers for light-driven killing of bacteria
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/108133
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6177443&blobtype=pdf
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