Cyclic-di-GMP signalling regulates motility and biofilm formation in <i>Bordetella bronchiseptica</i>

The signalling molecule bis-(39–59)-cyclic-dimeric guanosine monophosphate (c-di-GMP) is a central regulator of diverse cellular functions, including motility, biofilm formation, cell cycle progression and virulence, in bacteria. Multiple diguanylate cyclase and phosphodiesterasedomain-containing pr...

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Detalles Bibliográficos
Autores principales:	Sisti, Federico, Ha, Dae-Gon, O'Toole, George A., Hozbor, Daniela Flavia, Fernández, Julieta
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2013
Materias:	Ciencias Exactas Bordetella bronchiseptica
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/107998 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4085988&blobtype=pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-107998
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Bordetella bronchiseptica
spellingShingle	Ciencias Exactas Bordetella bronchiseptica Sisti, Federico Ha, Dae-Gon O'Toole, George A. Hozbor, Daniela Flavia Fernández, Julieta Cyclic-di-GMP signalling regulates motility and biofilm formation in <i>Bordetella bronchiseptica</i>
topic_facet	Ciencias Exactas Bordetella bronchiseptica
description	The signalling molecule bis-(39–59)-cyclic-dimeric guanosine monophosphate (c-di-GMP) is a central regulator of diverse cellular functions, including motility, biofilm formation, cell cycle progression and virulence, in bacteria. Multiple diguanylate cyclase and phosphodiesterasedomain-containing proteins (GGDEF and EAL/HD-GYP, respectively) modulate the levels of the second messenger c-di-GMP to transmit signals and obtain such specific cellular responses. In the genus Bordetella this c-di-GMP network is poorly studied. In this work, we evaluated the expression of two phenotypes in Bordetella bronchiseptica regulated by c-di-GMP, biofilm formation and motility, under the influence of ectopic expression of Pseudomonas aeruginosa proteins with EAL or GGDEF domains that regulates the c-di-GMP level. In agreement with previous reports for other bacteria, we observed that B. bronchiseptica is able to form biofilm and reduce its motility only when GGDEF domain protein is expressed. Moreover we identify a GGDEF domain protein (BB3576) with diguanylate cyclase activity that participates in motility and biofilm regulation in B. bronchiseptica. These results demonstrate for the first time, to our knowledge, the presence of c-di-GMP regulatory signalling in B. bronchiseptica.
format	Articulo Articulo
author	Sisti, Federico Ha, Dae-Gon O'Toole, George A. Hozbor, Daniela Flavia Fernández, Julieta
author_facet	Sisti, Federico Ha, Dae-Gon O'Toole, George A. Hozbor, Daniela Flavia Fernández, Julieta
author_sort	Sisti, Federico
title	Cyclic-di-GMP signalling regulates motility and biofilm formation in <i>Bordetella bronchiseptica</i>
title_short	Cyclic-di-GMP signalling regulates motility and biofilm formation in <i>Bordetella bronchiseptica</i>
title_full	Cyclic-di-GMP signalling regulates motility and biofilm formation in <i>Bordetella bronchiseptica</i>
title_fullStr	Cyclic-di-GMP signalling regulates motility and biofilm formation in <i>Bordetella bronchiseptica</i>
title_full_unstemmed	Cyclic-di-GMP signalling regulates motility and biofilm formation in <i>Bordetella bronchiseptica</i>
title_sort	cyclic-di-gmp signalling regulates motility and biofilm formation in <i>bordetella bronchiseptica</i>
publishDate	2013
url	http://sedici.unlp.edu.ar/handle/10915/107998 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4085988&blobtype=pdf
work_keys_str_mv	AT sistifederico cyclicdigmpsignallingregulatesmotilityandbiofilmformationinibordetellabronchisepticai AT hadaegon cyclicdigmpsignallingregulatesmotilityandbiofilmformationinibordetellabronchisepticai AT otoolegeorgea cyclicdigmpsignallingregulatesmotilityandbiofilmformationinibordetellabronchisepticai AT hozbordanielaflavia cyclicdigmpsignallingregulatesmotilityandbiofilmformationinibordetellabronchisepticai AT fernandezjulieta cyclicdigmpsignallingregulatesmotilityandbiofilmformationinibordetellabronchisepticai
bdutipo_str	Repositorios
_version_	1764820443874721794

Cyclic-di-GMP signalling regulates motility and biofilm formation in <i>Bordetella bronchiseptica</i>

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