Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>

<i>Bordetella pertussis</i>, a strictly human re-emerging pathogen and the causative agent of whooping cough, exploits a broad variety of virulence factors to establish efficient infection. Here, we used RNA sequencing to analyse the changes in gene expression profiles of human THP-1 mac...

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Autores principales: Petráčková, Denisa, Farman, Mariam R., Amman, Fabian, Linhartová, Irena, Dienstbier, Ana, Kumar, Dilip, Držmíšek, Jakub, Hofacker, Ivo, Rodríguez, María Eugenia, Večerek, Branislav
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Exactas
Biología
Bordetella pertussis
infection
macrophage
intracellular survival
host-pathogen interaction
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107929
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7237194&blobtype=pdf
https://www.tandfonline.com/doi/full/10.1080/15476286.2020.1727694
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107929
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Bordetella pertussis
infection
macrophage
intracellular survival
host-pathogen interaction
spellingShingle Ciencias Exactas
Biología
Bordetella pertussis
infection
macrophage
intracellular survival
host-pathogen interaction
Petráčková, Denisa
Farman, Mariam R.
Amman, Fabian
Linhartová, Irena
Dienstbier, Ana
Kumar, Dilip
Držmíšek, Jakub
Hofacker, Ivo
Rodríguez, María Eugenia
Večerek, Branislav
Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
topic_facet Ciencias Exactas
Biología
Bordetella pertussis
infection
macrophage
intracellular survival
host-pathogen interaction
description <i>Bordetella pertussis</i>, a strictly human re-emerging pathogen and the causative agent of whooping cough, exploits a broad variety of virulence factors to establish efficient infection. Here, we used RNA sequencing to analyse the changes in gene expression profiles of human THP-1 macrophages resulting from <i>B. pertussis</i> infection. In parallel, we attempted to determine the changes in intracellular <i>B. pertussis</i>-specific transcriptomic profiles resulting from interaction with macrophages. Our analysis revealed that global gene expression profiles in THP-1 macrophages are extensively rewired 6 h post-infection. Among the highly expressed genes, we identified those encoding cytokines, chemokines, and transcription regulators involved in the induction of the M1 and M2 macrophage polarization programmes. Notably, several host genes involved in the control of apoptosis and inflammation which are known to be hijacked by intracellular bacterial pathogens were overexpressed upon infection. Furthermore, <i>in silico</i> analyses identified large temporal changes in expression of specific gene subsets involved in signalling and metabolic pathways. Despite limited numbers of the bacterial reads, we observed reduced expression of majority of virulence factors and upregulation of several transcriptional regulators during infection suggesting that intracellular <i>B. pertussis</i> cells switch from virulent to avirulent phase and actively adapt to intracellular environment, respectively.
format Articulo
Articulo
author Petráčková, Denisa
Farman, Mariam R.
Amman, Fabian
Linhartová, Irena
Dienstbier, Ana
Kumar, Dilip
Držmíšek, Jakub
Hofacker, Ivo
Rodríguez, María Eugenia
Večerek, Branislav
author_facet Petráčková, Denisa
Farman, Mariam R.
Amman, Fabian
Linhartová, Irena
Dienstbier, Ana
Kumar, Dilip
Držmíšek, Jakub
Hofacker, Ivo
Rodríguez, María Eugenia
Večerek, Branislav
author_sort Petráčková, Denisa
title Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
title_short Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
title_full Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
title_fullStr Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
title_full_unstemmed Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
title_sort transcriptional profiling of human macrophages during infection with <i>bordetella pertussis</i>
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/107929
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7237194&blobtype=pdf
https://www.tandfonline.com/doi/full/10.1080/15476286.2020.1727694
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