Gene target discovery with network analysis in <i>Toxoplasma gondii</i>

Infectious diseases are of great relevance for global health, but needed drugs and vaccines have not been developed yet or are not efective in many cases. In fact, traditional scientifc approaches with intense focus on individual genes or proteins have not been successful in providing new treatments...

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Autores principales: Alonso, Andrés Mariano, Corvi, María M., Diambra, Luis Aníbal
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107924
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6345969&blobtype=pdf
https://www.nature.com/articles/s41598-018-36671-y
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id I19-R120-10915-107924
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Toxoplasma gondii
transcriptomic data
pathogens
spellingShingle Ciencias Exactas
Biología
Toxoplasma gondii
transcriptomic data
pathogens
Alonso, Andrés Mariano
Corvi, María M.
Diambra, Luis Aníbal
Gene target discovery with network analysis in <i>Toxoplasma gondii</i>
topic_facet Ciencias Exactas
Biología
Toxoplasma gondii
transcriptomic data
pathogens
description Infectious diseases are of great relevance for global health, but needed drugs and vaccines have not been developed yet or are not efective in many cases. In fact, traditional scientifc approaches with intense focus on individual genes or proteins have not been successful in providing new treatments. Hence, innovations in technology and computational methods provide new tools to further understand complex biological systems such as pathogen biology. In this paper, we apply a gene regulatory network approach to analyze transcriptomic data of the parasite <i>Toxoplasma gondii</i>. By means of an optimization procedure, the phenotypic transitions between the stages associated with the life cycle of <i>T. gondii</i> were embedded into the dynamics of a gene regulatory network. Thus, through this methodology we were able to reconstruct a gene regulatory network able to emulate the life cycle of the pathogen. The community network analysis has revealed that nodes of the network can be organized in seven communities which allow us to assign putative functions to 338 previously uncharacterized genes, 25 of which are predicted as new pathogenic factors. Furthermore, we identifed a small gene circuit that drives a series of phenotypic transitions that characterize the life cycle of this pathogen. These new fndings can contribute to the understanding of parasite pathogenesis.
format Articulo
Articulo
author Alonso, Andrés Mariano
Corvi, María M.
Diambra, Luis Aníbal
author_facet Alonso, Andrés Mariano
Corvi, María M.
Diambra, Luis Aníbal
author_sort Alonso, Andrés Mariano
title Gene target discovery with network analysis in <i>Toxoplasma gondii</i>
title_short Gene target discovery with network analysis in <i>Toxoplasma gondii</i>
title_full Gene target discovery with network analysis in <i>Toxoplasma gondii</i>
title_fullStr Gene target discovery with network analysis in <i>Toxoplasma gondii</i>
title_full_unstemmed Gene target discovery with network analysis in <i>Toxoplasma gondii</i>
title_sort gene target discovery with network analysis in <i>toxoplasma gondii</i>
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/107924
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6345969&blobtype=pdf
https://www.nature.com/articles/s41598-018-36671-y
work_keys_str_mv AT alonsoandresmariano genetargetdiscoverywithnetworkanalysisinitoxoplasmagondiii
AT corvimariam genetargetdiscoverywithnetworkanalysisinitoxoplasmagondiii
AT diambraluisanibal genetargetdiscoverywithnetworkanalysisinitoxoplasmagondiii
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