Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades

Structural knowledge of gastropod hemocyanins is scarce. To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, <i>Pomacea canaliculata</i> (PcH). Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with o...

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Detalles Bibliográficos
Autores principales:	Chiumiento, Ignacio Rafael, Ituarte, Santiago, Sun, Jin, Qiu, Jian Wen, Heras, Horacio, Dreon, Marcos Sebastián
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2020
Materias:	Ciencias Exactas Ciencias Naturales Pomacea canaliculata Gastropod hemocyanins Gene structure study
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/107884 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6992001&blobtype=pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-107884
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Ciencias Naturales Pomacea canaliculata Gastropod hemocyanins Gene structure study
spellingShingle	Ciencias Exactas Ciencias Naturales Pomacea canaliculata Gastropod hemocyanins Gene structure study Chiumiento, Ignacio Rafael Ituarte, Santiago Sun, Jin Qiu, Jian Wen Heras, Horacio Dreon, Marcos Sebastián Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
topic_facet	Ciencias Exactas Ciencias Naturales Pomacea canaliculata Gastropod hemocyanins Gene structure study
description	Structural knowledge of gastropod hemocyanins is scarce. To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, <i>Pomacea canaliculata</i> (PcH). Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3. Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs). Correspondingly, genes are organized in eight FUs coding regions. All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods. Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tridecameric cylinders the most abundant ones. PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid. Terminal β-N-GlcNAc residues and nonreducing terminal α-GalNAc are also present. The molecule lacks O-linked glycosylation but presents the T-antigen (Gal-β1,3-GalNAc). Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins. This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.
format	Articulo Articulo
author	Chiumiento, Ignacio Rafael Ituarte, Santiago Sun, Jin Qiu, Jian Wen Heras, Horacio Dreon, Marcos Sebastián
author_facet	Chiumiento, Ignacio Rafael Ituarte, Santiago Sun, Jin Qiu, Jian Wen Heras, Horacio Dreon, Marcos Sebastián
author_sort	Chiumiento, Ignacio Rafael
title	Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
title_short	Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
title_full	Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
title_fullStr	Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
title_full_unstemmed	Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
title_sort	hemocyanin of the caenogastropod <i>pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
publishDate	2020
url	http://sedici.unlp.edu.ar/handle/10915/107884 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6992001&blobtype=pdf
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Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades

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