Comparative Analysis of the Biosimilar and Innovative G-CSF Modulated Pathways on Umbilical Cord Blood-Derived Mononuclear Cells
Biosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro...
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| Autores principales: | , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2020
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/107843 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7088127&blobtype=pdf |
| Aporte de: |
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I19-R120-10915-107843 |
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dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Biología G-CSF Innovator Biosimilar Umbilical cord blood Transcriptomics |
| spellingShingle |
Biología G-CSF Innovator Biosimilar Umbilical cord blood Transcriptomics Avila-Portillo, L. M. Aristizabal, F. Perdomo, S. Riveros, A. Ospino, B. Avila, J. P. Butti, Matías A. Abba, Martín Carlos Comparative Analysis of the Biosimilar and Innovative G-CSF Modulated Pathways on Umbilical Cord Blood-Derived Mononuclear Cells |
| topic_facet |
Biología G-CSF Innovator Biosimilar Umbilical cord blood Transcriptomics |
| description |
Biosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways.
Umbilical cord blood cells–derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs.
In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses. |
| format |
Articulo Articulo |
| author |
Avila-Portillo, L. M. Aristizabal, F. Perdomo, S. Riveros, A. Ospino, B. Avila, J. P. Butti, Matías A. Abba, Martín Carlos |
| author_facet |
Avila-Portillo, L. M. Aristizabal, F. Perdomo, S. Riveros, A. Ospino, B. Avila, J. P. Butti, Matías A. Abba, Martín Carlos |
| author_sort |
Avila-Portillo, L. M. |
| title |
Comparative Analysis of the Biosimilar and Innovative G-CSF Modulated Pathways on Umbilical Cord Blood-Derived Mononuclear Cells |
| title_short |
Comparative Analysis of the Biosimilar and Innovative G-CSF Modulated Pathways on Umbilical Cord Blood-Derived Mononuclear Cells |
| title_full |
Comparative Analysis of the Biosimilar and Innovative G-CSF Modulated Pathways on Umbilical Cord Blood-Derived Mononuclear Cells |
| title_fullStr |
Comparative Analysis of the Biosimilar and Innovative G-CSF Modulated Pathways on Umbilical Cord Blood-Derived Mononuclear Cells |
| title_full_unstemmed |
Comparative Analysis of the Biosimilar and Innovative G-CSF Modulated Pathways on Umbilical Cord Blood-Derived Mononuclear Cells |
| title_sort |
comparative analysis of the biosimilar and innovative g-csf modulated pathways on umbilical cord blood-derived mononuclear cells |
| publishDate |
2020 |
| url |
http://sedici.unlp.edu.ar/handle/10915/107843 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7088127&blobtype=pdf |
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