A Rhizobium meliloti lipopolysaccharide mutant altered in competitiveness for nodulation of alfalfa

A transposon Tn5-induced mutant of Rhizobium meliloti Rm2011, designated Rm6963, showed a rough colony morphology on rich and minimal media and an altered lipopolysaccharide (LPS). Major differences from the wild-type LPS were observed in (i) hexose and 2-keto-3-deoxyoctonate elution profiles of cru...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Lagares, Antonio, Caetano Anollés, Gustavo, Niehaus, K., Lorenzen, J., Ljunggren, H. D, Pühler, A, Favelukes, Gabriel
Formato: Articulo
Lenguaje:Español
Publicado: 1992
Materias:
Ciencias Exactas
Rhizobium meliloti
Alfalfa
Lipopolysaccharide
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107829
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC207132&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107829
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Ciencias Exactas
Rhizobium meliloti
Alfalfa
Lipopolysaccharide
spellingShingle Ciencias Exactas
Rhizobium meliloti
Alfalfa
Lipopolysaccharide
Lagares, Antonio
Caetano Anollés, Gustavo
Niehaus, K.
Lorenzen, J.
Ljunggren, H. D
Pühler, A
Favelukes, Gabriel
A Rhizobium meliloti lipopolysaccharide mutant altered in competitiveness for nodulation of alfalfa
topic_facet Ciencias Exactas
Rhizobium meliloti
Alfalfa
Lipopolysaccharide
description A transposon Tn5-induced mutant of Rhizobium meliloti Rm2011, designated Rm6963, showed a rough colony morphology on rich and minimal media and an altered lipopolysaccharide (LPS). Major differences from the wild-type LPS were observed in (i) hexose and 2-keto-3-deoxyoctonate elution profiles of crude phenol extracts chromatographed in Sepharose CL-4B, (ii) silver-stained sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis patterns of crude and purified LPS fractions, and (iii) immunoreactivities otherwise present in purified LPS of the parental strain Rm2011. In addition, Rm6963 lost the ability to grow in Luria-Bertani medium containing the hydrophobic compounds sodium deoxycholate or SDS and showed a decrease in survival in TY medium supplemented with high calcium concentrations. The mutant also had altered symbiotic properties. Rm6963 formed nodules that fixed nitrogen but showed a delayed or even reduced ability to nodulate the primary root of alfalfa without showing changes in the position of nodule distribution profiles along the roots. Furthermore, 2 to 3 weeks after inoculation, plants nodulated by Rm6963 were smaller than control plants inoculated with wild-type bacteria in correlation with a transient decrease in nitrogen fixation. In most experiments, the plants recovered later by expressing a full nitrogen-fixing phenotype and developing an abnormally high number of small nodules in lateral roots after 1 month. Rm6963 was also deficient in the ability to compete for nodulation. In coinoculation experiments with equal bacterial numbers of both mutant and wild-type rhizobia, only the parent was recovered from the uppermost root nodules. A strain ratio of approximately 100 to 1 favoring the mutant was necessary to obtain an equal ratio (1:1) of nodule occupancy. These results show that alterations in Rm6963 which include LPS changes lead to an altered symbiotic phenotype during the association with alfalfa that affects the timing of nodule emergence, the progress of nitrogen fixation, and the strain competitiveness for nodulation.
format Articulo
Articulo
author Lagares, Antonio
Caetano Anollés, Gustavo
Niehaus, K.
Lorenzen, J.
Ljunggren, H. D
Pühler, A
Favelukes, Gabriel
author_facet Lagares, Antonio
Caetano Anollés, Gustavo
Niehaus, K.
Lorenzen, J.
Ljunggren, H. D
Pühler, A
Favelukes, Gabriel
author_sort Lagares, Antonio
title A Rhizobium meliloti lipopolysaccharide mutant altered in competitiveness for nodulation of alfalfa
title_short A Rhizobium meliloti lipopolysaccharide mutant altered in competitiveness for nodulation of alfalfa
title_full A Rhizobium meliloti lipopolysaccharide mutant altered in competitiveness for nodulation of alfalfa
title_fullStr A Rhizobium meliloti lipopolysaccharide mutant altered in competitiveness for nodulation of alfalfa
title_full_unstemmed A Rhizobium meliloti lipopolysaccharide mutant altered in competitiveness for nodulation of alfalfa
title_sort rhizobium meliloti lipopolysaccharide mutant altered in competitiveness for nodulation of alfalfa
publishDate 1992
url http://sedici.unlp.edu.ar/handle/10915/107829
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC207132&blobtype=pdf
work_keys_str_mv AT lagaresantonio arhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT caetanoanollesgustavo arhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT niehausk arhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT lorenzenj arhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT ljunggrenhd arhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT puhlera arhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT favelukesgabriel arhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT lagaresantonio rhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT caetanoanollesgustavo rhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT niehausk rhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT lorenzenj rhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT ljunggrenhd rhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT puhlera rhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
AT favelukesgabriel rhizobiummelilotilipopolysaccharidemutantalteredincompetitivenessfornodulationofalfalfa
bdutipo_str Repositorios
_version_ 1764820443679686656

Ejemplares similares