Characterization of FliL Proteins in <i>Bradyrhizobium diazoefficiens</i> : Lateral FliL Supports Swimming Motility, and Subpolar FliL Modulates the Lateral Flagellar System

<i>Bradyrhizobium diazoefficiens</i> is a soil alphaproteobacterium that possesses two evolutionarily distinct flagellar systems, a constitutive subpolar flagellum and inducible lateral flagella that, depending on the carbon source, may be expressed simultaneously in liquid medium and us...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Mengucci, Florencia, Dardis, Carolina, Mongiardini, Elías Javier, Althabegoiti, María Julia, Partridge, Jonathan D., Kojima, Seiji, Homma, Michio, Quelas, Juan Ignacio, Lodeiro, Aníbal Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Exactas
Bradyrhizobium
FliL
Flagella
Swimming
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107803
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7015707&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107803
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bradyrhizobium
FliL
Flagella
Swimming
spellingShingle Ciencias Exactas
Bradyrhizobium
FliL
Flagella
Swimming
Mengucci, Florencia
Dardis, Carolina
Mongiardini, Elías Javier
Althabegoiti, María Julia
Partridge, Jonathan D.
Kojima, Seiji
Homma, Michio
Quelas, Juan Ignacio
Lodeiro, Aníbal Roberto
Characterization of FliL Proteins in <i>Bradyrhizobium diazoefficiens</i> : Lateral FliL Supports Swimming Motility, and Subpolar FliL Modulates the Lateral Flagellar System
topic_facet Ciencias Exactas
Bradyrhizobium
FliL
Flagella
Swimming
description <i>Bradyrhizobium diazoefficiens</i> is a soil alphaproteobacterium that possesses two evolutionarily distinct flagellar systems, a constitutive subpolar flagellum and inducible lateral flagella that, depending on the carbon source, may be expressed simultaneously in liquid medium and used interactively for swimming. In each system, more than 30 genes encode the flagellar proteins, most of which are well characterized. Among the exceptions is FliL, which has been scarcely studied in alphaproteobacteria and whose function in other bacterial classes is somewhat controversial. Because each <i>B. diazoefficiens</i> flagellar system contains its own <i>fliL</i> paralog, we obtained the respective deletions ΔfliL<sub>S</sub> (subpolar) and ΔfliL<sub>L</sub> (lateral) to study their functions in swimming. We determined that FliL<sub>L</sub> was essential for lateral flagellum-driven motility. FliL<sub>S</sub> was dispensable for swimming in either liquid or semisolid medium; however, it was found to play a crucial role in upregulation of the lateral flagellum regulon under conditions of increased viscosity/flagellar load. Therefore, although FliL<sub>S</sub> seems to be not essential for swimming, it may participate in a mechanosensor complex that controls lateral flagellum induction
format Articulo
Articulo
author Mengucci, Florencia
Dardis, Carolina
Mongiardini, Elías Javier
Althabegoiti, María Julia
Partridge, Jonathan D.
Kojima, Seiji
Homma, Michio
Quelas, Juan Ignacio
Lodeiro, Aníbal Roberto
author_facet Mengucci, Florencia
Dardis, Carolina
Mongiardini, Elías Javier
Althabegoiti, María Julia
Partridge, Jonathan D.
Kojima, Seiji
Homma, Michio
Quelas, Juan Ignacio
Lodeiro, Aníbal Roberto
author_sort Mengucci, Florencia
title Characterization of FliL Proteins in <i>Bradyrhizobium diazoefficiens</i> : Lateral FliL Supports Swimming Motility, and Subpolar FliL Modulates the Lateral Flagellar System
title_short Characterization of FliL Proteins in <i>Bradyrhizobium diazoefficiens</i> : Lateral FliL Supports Swimming Motility, and Subpolar FliL Modulates the Lateral Flagellar System
title_full Characterization of FliL Proteins in <i>Bradyrhizobium diazoefficiens</i> : Lateral FliL Supports Swimming Motility, and Subpolar FliL Modulates the Lateral Flagellar System
title_fullStr Characterization of FliL Proteins in <i>Bradyrhizobium diazoefficiens</i> : Lateral FliL Supports Swimming Motility, and Subpolar FliL Modulates the Lateral Flagellar System
title_full_unstemmed Characterization of FliL Proteins in <i>Bradyrhizobium diazoefficiens</i> : Lateral FliL Supports Swimming Motility, and Subpolar FliL Modulates the Lateral Flagellar System
title_sort characterization of flil proteins in <i>bradyrhizobium diazoefficiens</i> : lateral flil supports swimming motility, and subpolar flil modulates the lateral flagellar system
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/107803
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7015707&blobtype=pdf
work_keys_str_mv AT mengucciflorencia characterizationofflilproteinsinibradyrhizobiumdiazoefficiensilateralflilsupportsswimmingmotilityandsubpolarflilmodulatesthelateralflagellarsystem
AT dardiscarolina characterizationofflilproteinsinibradyrhizobiumdiazoefficiensilateralflilsupportsswimmingmotilityandsubpolarflilmodulatesthelateralflagellarsystem
AT mongiardinieliasjavier characterizationofflilproteinsinibradyrhizobiumdiazoefficiensilateralflilsupportsswimmingmotilityandsubpolarflilmodulatesthelateralflagellarsystem
AT althabegoitimariajulia characterizationofflilproteinsinibradyrhizobiumdiazoefficiensilateralflilsupportsswimmingmotilityandsubpolarflilmodulatesthelateralflagellarsystem
AT partridgejonathand characterizationofflilproteinsinibradyrhizobiumdiazoefficiensilateralflilsupportsswimmingmotilityandsubpolarflilmodulatesthelateralflagellarsystem
AT kojimaseiji characterizationofflilproteinsinibradyrhizobiumdiazoefficiensilateralflilsupportsswimmingmotilityandsubpolarflilmodulatesthelateralflagellarsystem
AT hommamichio characterizationofflilproteinsinibradyrhizobiumdiazoefficiensilateralflilsupportsswimmingmotilityandsubpolarflilmodulatesthelateralflagellarsystem
AT quelasjuanignacio characterizationofflilproteinsinibradyrhizobiumdiazoefficiensilateralflilsupportsswimmingmotilityandsubpolarflilmodulatesthelateralflagellarsystem
AT lodeiroanibalroberto characterizationofflilproteinsinibradyrhizobiumdiazoefficiensilateralflilsupportsswimmingmotilityandsubpolarflilmodulatesthelateralflagellarsystem
bdutipo_str Repositorios
_version_ 1764820443634597891

Ejemplares similares