Melaleuca armillaris Essential Oil in Combination With Rifaximin Against Staphylococcus aureus Isolated of Dairy Cows

Staphylococcus aureus is the major subclinical mastitis-causing pathogen in dairy cows. In some European and Latin American countries, rifaximin (RIF) is a commonly used therapy at drying off. Phytotherapeutics are alternatives for the treatment of infectious diseases. Melaleuca armillaris essentia...

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Autores principales: Buldain, Daniel Cornelio, Gortari Castillo, Lihuel, Buchamer, Andrea Verónica, Aliverti, Florencia, Bandoni, Arnaldo, Marchetti, María Laura, Mestorino, Olga Nora
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Veterinarias
Staphylococcus aureus
Dairy cows
Melaleuca armillaris
Essential oil
Rifaximin
Checkerboard
Synergism
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107793
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7373747&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107793
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Staphylococcus aureus
Dairy cows
Melaleuca armillaris
Essential oil
Rifaximin
Checkerboard
Synergism
spellingShingle Ciencias Veterinarias
Staphylococcus aureus
Dairy cows
Melaleuca armillaris
Essential oil
Rifaximin
Checkerboard
Synergism
Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Aliverti, Florencia
Bandoni, Arnaldo
Marchetti, María Laura
Mestorino, Olga Nora
Melaleuca armillaris Essential Oil in Combination With Rifaximin Against Staphylococcus aureus Isolated of Dairy Cows
topic_facet Ciencias Veterinarias
Staphylococcus aureus
Dairy cows
Melaleuca armillaris
Essential oil
Rifaximin
Checkerboard
Synergism
description Staphylococcus aureus is the major subclinical mastitis-causing pathogen in dairy cows. In some European and Latin American countries, rifaximin (RIF) is a commonly used therapy at drying off. Phytotherapeutics are alternatives for the treatment of infectious diseases. Melaleuca armillaris essential oil (EO) has been reported as a good antimicrobial against S. aureus. The aim of this work was to investigate, in vitro, the combined effect of EO and RIF to identify a synergic interaction against S. aureus in order to obtain enough information for subsequent pharmacokinetic/pharmacodynamic studies. The minimum inhibitory concentrations (MIC) for RIF, EO, and combinations of these against S. aureus strains were determined at pH 7.4, 6.5, and 5.0, representing intracellular conditions where S. aureus is usually located. The fractional inhibitory concentration index (FIC) and the index of antibacterial activity (E) were evaluated. The MIC of EO at pH 7.4 was 25–12.5 µL/mL and decreased with the acidity of the medium. RIF presented a high antimicrobial activity (0.032µg/mL) against S. aureus regardless of the pH conditions. Combining RIF with EO, we found a synergic effect. A mix of 0.004µg/mL of RIF and 12.5 µL/mL of EO led to a virtual eradication effect against wild-type strains at pH 7.4. Media acidification improves the EO/RIF activity, so EO would be a good adjuvant for RIF to treat staphylococcal infections and decrease antimicrobial resistance.
format Articulo
Articulo
author Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Aliverti, Florencia
Bandoni, Arnaldo
Marchetti, María Laura
Mestorino, Olga Nora
author_facet Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Aliverti, Florencia
Bandoni, Arnaldo
Marchetti, María Laura
Mestorino, Olga Nora
author_sort Buldain, Daniel Cornelio
title Melaleuca armillaris Essential Oil in Combination With Rifaximin Against Staphylococcus aureus Isolated of Dairy Cows
title_short Melaleuca armillaris Essential Oil in Combination With Rifaximin Against Staphylococcus aureus Isolated of Dairy Cows
title_full Melaleuca armillaris Essential Oil in Combination With Rifaximin Against Staphylococcus aureus Isolated of Dairy Cows
title_fullStr Melaleuca armillaris Essential Oil in Combination With Rifaximin Against Staphylococcus aureus Isolated of Dairy Cows
title_full_unstemmed Melaleuca armillaris Essential Oil in Combination With Rifaximin Against Staphylococcus aureus Isolated of Dairy Cows
title_sort melaleuca armillaris essential oil in combination with rifaximin against staphylococcus aureus isolated of dairy cows
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/107793
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7373747&blobtype=pdf
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