Synergistic effect of carboxypterin and methylene blue applied to antimicrobial photodynamic therapy against mature biofilm of Klebsiella pneumoniae

The control of multidrug-resistant (MDR) bacteria is a growing public health problem, and new strategies are urgently needed for the control of the infections caused by these microorganisms. Notoriously, some MDR microorganisms generate complex structures or biofilms, which adhere to surfaces and co...

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Detalles Bibliográficos
Autores principales:	Gaspar Tosato, Maira, Schilardi, Patricia Laura, Fernández Lorenzo de Mele, Mónica Alicia, Thomas, Andrés Héctor, Lorente, Carolina, Miñán, Alejandro
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2020
Materias:	Ciencias Exactas Microbiology Antibiotic resistant bacteria Bacteria Bacteriology Biofilms Medical microbiology Photochemistry Antimicrobial photodynamic therapy Biofilm Reactive oxygen species Methylene blue Carboxypterin Klebsiella pneumoniae Multidrug resistance
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/107789 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7075992&blobtype=pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-107789
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Microbiology Antibiotic resistant bacteria Bacteria Bacteriology Biofilms Medical microbiology Photochemistry Antimicrobial photodynamic therapy Biofilm Reactive oxygen species Methylene blue Carboxypterin Klebsiella pneumoniae Multidrug resistance
spellingShingle	Ciencias Exactas Microbiology Antibiotic resistant bacteria Bacteria Bacteriology Biofilms Medical microbiology Photochemistry Antimicrobial photodynamic therapy Biofilm Reactive oxygen species Methylene blue Carboxypterin Klebsiella pneumoniae Multidrug resistance Gaspar Tosato, Maira Schilardi, Patricia Laura Fernández Lorenzo de Mele, Mónica Alicia Thomas, Andrés Héctor Lorente, Carolina Miñán, Alejandro Synergistic effect of carboxypterin and methylene blue applied to antimicrobial photodynamic therapy against mature biofilm of Klebsiella pneumoniae
topic_facet	Ciencias Exactas Microbiology Antibiotic resistant bacteria Bacteria Bacteriology Biofilms Medical microbiology Photochemistry Antimicrobial photodynamic therapy Biofilm Reactive oxygen species Methylene blue Carboxypterin Klebsiella pneumoniae Multidrug resistance
description	The control of multidrug-resistant (MDR) bacteria is a growing public health problem, and new strategies are urgently needed for the control of the infections caused by these microorganisms. Notoriously, some MDR microorganisms generate complex structures or biofilms, which adhere to surfaces and confer extraordinary resistance properties that are fundamental challenges to control infections. One of the promising strategies for the control of MDR bacteria is antimicrobial photodynamic therapy (aPDT), which takes advantage of suitable photosensitizers (PS), oxygen and radiation to eradicate microorganisms by the generation of highly reactive species, including reactive oxygen species (ROS) that cause cytotoxic damage and cell death. Habitual aPDT treatments use only methylene blue (MB), but MDR microorganism eradication is not completely achieved. The key result of this study revealed that a combination of two known PSs, 6-carboxypterin (Cap, 100 μM) and MB (2.5–10 μM) exposed to ultraviolet and visible radiation, presents a synergistic effect on the eradication of a MDR <i>Klebsiella pneumoniae</i> strain. Similar effect was observed when the treatment was performed either with planktonic or biofilm growing cells. Moreover, it was found that after treatment the killing action continues in the absence of irradiation leading to the eradication of the microorganisms growing in biofilm. Therefore, the combined aPDT represents a promising strategy for the management of clinical contact surfaces, disinfection of surgical instruments, biofouling and even antimicrobial wastewater treatment.
format	Articulo Articulo
author	Gaspar Tosato, Maira Schilardi, Patricia Laura Fernández Lorenzo de Mele, Mónica Alicia Thomas, Andrés Héctor Lorente, Carolina Miñán, Alejandro
author_facet	Gaspar Tosato, Maira Schilardi, Patricia Laura Fernández Lorenzo de Mele, Mónica Alicia Thomas, Andrés Héctor Lorente, Carolina Miñán, Alejandro
author_sort	Gaspar Tosato, Maira
title	Synergistic effect of carboxypterin and methylene blue applied to antimicrobial photodynamic therapy against mature biofilm of Klebsiella pneumoniae
title_short	Synergistic effect of carboxypterin and methylene blue applied to antimicrobial photodynamic therapy against mature biofilm of Klebsiella pneumoniae
title_full	Synergistic effect of carboxypterin and methylene blue applied to antimicrobial photodynamic therapy against mature biofilm of Klebsiella pneumoniae
title_fullStr	Synergistic effect of carboxypterin and methylene blue applied to antimicrobial photodynamic therapy against mature biofilm of Klebsiella pneumoniae
title_full_unstemmed	Synergistic effect of carboxypterin and methylene blue applied to antimicrobial photodynamic therapy against mature biofilm of Klebsiella pneumoniae
title_sort	synergistic effect of carboxypterin and methylene blue applied to antimicrobial photodynamic therapy against mature biofilm of klebsiella pneumoniae
publishDate	2020
url	http://sedici.unlp.edu.ar/handle/10915/107789 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7075992&blobtype=pdf
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Synergistic effect of carboxypterin and methylene blue applied to antimicrobial photodynamic therapy against mature biofilm of Klebsiella pneumoniae

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