Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells

Glycerol-3-phosphate acyltransferase-2 is a member of “cancer-testis gene” family. Initially linked to lipid metabolism, this gene has been recently found involved also in PIWI-interacting RNAs biogenesis in germline stem cells. To investigate its role in piRNA metabolism in cancer, the gene was sil...

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Autores principales: Lacunza, Ezequiel, Montanaro, Mauro Aldo, Salvati, Annamaria, Memoli, Domenico, Rizzo, Francesca, Henning, María Florencia, Quiroga, Ivana Yoseli, Guillou, Hervé, Abba, Martín Carlos, González Baró, María del Rosario, Weisz, Alessandro, Pellón Maisón, Magalí
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Médicas
GPAT2
breast cancer
piRNAs
tRNA derived fragments
small non-coding RNAs
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107772
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6021339&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107772
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
GPAT2
breast cancer
piRNAs
tRNA derived fragments
small non-coding RNAs
spellingShingle Ciencias Médicas
GPAT2
breast cancer
piRNAs
tRNA derived fragments
small non-coding RNAs
Lacunza, Ezequiel
Montanaro, Mauro Aldo
Salvati, Annamaria
Memoli, Domenico
Rizzo, Francesca
Henning, María Florencia
Quiroga, Ivana Yoseli
Guillou, Hervé
Abba, Martín Carlos
González Baró, María del Rosario
Weisz, Alessandro
Pellón Maisón, Magalí
Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
topic_facet Ciencias Médicas
GPAT2
breast cancer
piRNAs
tRNA derived fragments
small non-coding RNAs
description Glycerol-3-phosphate acyltransferase-2 is a member of “cancer-testis gene” family. Initially linked to lipid metabolism, this gene has been recently found involved also in PIWI-interacting RNAs biogenesis in germline stem cells. To investigate its role in piRNA metabolism in cancer, the gene was silenced in MDA-MB-231 breast cancer cells and small RNA sequencing was applied. PIWI-interacting RNAs and tRNA-derived fragments expression profiles showed changes following GPAT2 silencing. Interestingly, a marked shift in length distribution for both small RNAs was detected in GPAT2-silenced cells. Most downregulated PIWI-interacting RNAs are single copy in the genome, intragenic, hosted in snoRNAs and previously found to be upregulated in cancer cells. Putative targets of these PIWI-interacting RNAs are linked to lipid metabolism. Downregulated tRNA derived fragments derived from, socalled ‘differentiation tRNAs’, whereas upregulated ones derived from proliferationlinked tRNAs. miRNA amounts decrease after Glycerol-3-phosphate acyltransferase-2 silencing and functional enrichment analysis of deregulated miRNA putative targets point to mitochondrial biogenesis, IGF1R signaling and oxidative metabolism of lipids and lipoproteins. In addition, miRNAs known to be overexpressed in breast cancer tumors with poor prognosis where found downregulated in GPAT2-silenced cells. In conclusion, GPAT2 silencing quantitatively and qualitatively affects the population of PIWI-interacting RNAs, tRNA derived fragments and miRNAs which, in combination, result in a more differentiated cancer cell phenotype.
format Articulo
Articulo
author Lacunza, Ezequiel
Montanaro, Mauro Aldo
Salvati, Annamaria
Memoli, Domenico
Rizzo, Francesca
Henning, María Florencia
Quiroga, Ivana Yoseli
Guillou, Hervé
Abba, Martín Carlos
González Baró, María del Rosario
Weisz, Alessandro
Pellón Maisón, Magalí
author_facet Lacunza, Ezequiel
Montanaro, Mauro Aldo
Salvati, Annamaria
Memoli, Domenico
Rizzo, Francesca
Henning, María Florencia
Quiroga, Ivana Yoseli
Guillou, Hervé
Abba, Martín Carlos
González Baró, María del Rosario
Weisz, Alessandro
Pellón Maisón, Magalí
author_sort Lacunza, Ezequiel
title Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
title_short Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
title_full Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
title_fullStr Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
title_full_unstemmed Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
title_sort small non-coding rna landscape is modified by gpat2 silencing in mda-mb-231 cells
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/107772
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6021339&blobtype=pdf
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