Intranasal acellular pertussis vaccine provides mucosal immunity and protects mice from <i>Bordetella pertussis</i>

Current acellular pertussis vaccines fall short of optimal protection against the human respiratory pathogen <i>Bordetella pertussis</i> resulting in increased incidence of a previously controlled vaccine- preventable disease. Natural infection is known to induce a protective mucosal imm...

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Autores principales:	Boehm, Dylan T., Wolf, M. Allison, Hall, Jesse M., Wong, Ting Y., Sen Kilic, Emel, Basinger, Hayden D., Dziadowicz, Sebastian A., Gutierrez, María de la Paz, Blackwood, Catherine B., Bradford, Shelby D., Begley, Katherine A., Witt, William T., Varney, Melinda E., Barbier, Mariette, Damron, F. Heath
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2019
Materias:	Ciencias Exactas Biología adjuvants bacterial infection immunology protein vaccines vaccines
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/107765 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6776550&blobtype=pdf https://www.nature.com/articles/s41541-019-0136-2
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-107765
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Biología adjuvants bacterial infection immunology protein vaccines vaccines
spellingShingle	Ciencias Exactas Biología adjuvants bacterial infection immunology protein vaccines vaccines Boehm, Dylan T. Wolf, M. Allison Hall, Jesse M. Wong, Ting Y. Sen Kilic, Emel Basinger, Hayden D. Dziadowicz, Sebastian A. Gutierrez, María de la Paz Blackwood, Catherine B. Bradford, Shelby D. Begley, Katherine A. Witt, William T. Varney, Melinda E. Barbier, Mariette Damron, F. Heath Intranasal acellular pertussis vaccine provides mucosal immunity and protects mice from <i>Bordetella pertussis</i>
topic_facet	Ciencias Exactas Biología adjuvants bacterial infection immunology protein vaccines vaccines
description	Current acellular pertussis vaccines fall short of optimal protection against the human respiratory pathogen <i>Bordetella pertussis</i> resulting in increased incidence of a previously controlled vaccine- preventable disease. Natural infection is known to induce a protective mucosal immunity. Therefore, in this study, we aimed to use acellular pertussis vaccines to recapitulate these mucosal immune responses. We utilized a murine immunization and challenge model to characterize the efficacy of intranasal immunization (IN) with DTaP vaccine or DTaP vaccine supplemented with curdlan, a known Th1/Th17 promoting adjuvant. Protection from IN delivered DTaP was compared to protection mediated by intraperitoneal injection of DTaP and whole-cell pertussis vaccines. We tracked fluorescently labeled DTaP after immunization and detected that DTaP localized preferentially in the lungs while DTaP with curdlan was predominantly in the nasal turbinates. IN immunization with DTaP, with or without curdlan adjuvant, resulted in anti-<i>B. pertussis</i> and anti-pertussis toxin IgG titers at the same level as intraperitoneally administered DTaP. IN immunization was able to protect against <i>B. pertussis</i> challenge and we observed decreased pulmonary pro-inflammatory cytokines, neutrophil infiltrates in the lung, and bacterial burden in the upper and lower respiratory tract at day 3 post challenge. Furthermore, IN immunization with DTaP triggered mucosal immune responses such as production of <i>B. pertussis</i>-specific IgA, and increased IL-17A. Together, the induction of a mucosal immune response and humoral antibody-mediated protection associated with an IN administered DTaP and curdlan adjuvant warrant further exploration as a pertussis vaccine candidate formulation.
format	Articulo Articulo
author	Boehm, Dylan T. Wolf, M. Allison Hall, Jesse M. Wong, Ting Y. Sen Kilic, Emel Basinger, Hayden D. Dziadowicz, Sebastian A. Gutierrez, María de la Paz Blackwood, Catherine B. Bradford, Shelby D. Begley, Katherine A. Witt, William T. Varney, Melinda E. Barbier, Mariette Damron, F. Heath
author_facet	Boehm, Dylan T. Wolf, M. Allison Hall, Jesse M. Wong, Ting Y. Sen Kilic, Emel Basinger, Hayden D. Dziadowicz, Sebastian A. Gutierrez, María de la Paz Blackwood, Catherine B. Bradford, Shelby D. Begley, Katherine A. Witt, William T. Varney, Melinda E. Barbier, Mariette Damron, F. Heath
author_sort	Boehm, Dylan T.
title	Intranasal acellular pertussis vaccine provides mucosal immunity and protects mice from <i>Bordetella pertussis</i>
title_short	Intranasal acellular pertussis vaccine provides mucosal immunity and protects mice from <i>Bordetella pertussis</i>
title_full	Intranasal acellular pertussis vaccine provides mucosal immunity and protects mice from <i>Bordetella pertussis</i>
title_fullStr	Intranasal acellular pertussis vaccine provides mucosal immunity and protects mice from <i>Bordetella pertussis</i>
title_full_unstemmed	Intranasal acellular pertussis vaccine provides mucosal immunity and protects mice from <i>Bordetella pertussis</i>
title_sort	intranasal acellular pertussis vaccine provides mucosal immunity and protects mice from <i>bordetella pertussis</i>
publishDate	2019
url	http://sedici.unlp.edu.ar/handle/10915/107765 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6776550&blobtype=pdf https://www.nature.com/articles/s41541-019-0136-2
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bdutipo_str	Repositorios
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Intranasal acellular pertussis vaccine provides mucosal immunity and protects mice from <i>Bordetella pertussis</i>

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