<i>Bordetella pertussis</i> Can Be Motile and Express Flagellum-Like Structures

Bordetella bronchiseptica encodes and expresses a flagellar apparatus. In contrast, Bordetella pertussis, the causative agent of whooping cough, has historically been described as a nonmotile and nonflagellated organism. The previous statements that B. pertussis was a nonmotile organism were consist...

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Autores principales:	Hoffman, Casandra L., Gonyar, Laura A., Zacca, Federico, Sisti, Federico, Fernández, Julieta, Wong, Ting, Damron, F. Heath, Hewlett, Erik L.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2019
Materias:	Ciencias Exactas Bordetella Bordetella bronchiseptica Bordetella pertussis flagella flagellar motility flagellar structure motility
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/107762 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6520453&blobtype=pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-107762
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Bordetella Bordetella bronchiseptica Bordetella pertussis flagella flagellar motility flagellar structure motility
spellingShingle	Ciencias Exactas Bordetella Bordetella bronchiseptica Bordetella pertussis flagella flagellar motility flagellar structure motility Hoffman, Casandra L. Gonyar, Laura A. Zacca, Federico Sisti, Federico Fernández, Julieta Wong, Ting Damron, F. Heath Hewlett, Erik L. <i>Bordetella pertussis</i> Can Be Motile and Express Flagellum-Like Structures
topic_facet	Ciencias Exactas Bordetella Bordetella bronchiseptica Bordetella pertussis flagella flagellar motility flagellar structure motility
description	Bordetella bronchiseptica encodes and expresses a flagellar apparatus. In contrast, Bordetella pertussis, the causative agent of whooping cough, has historically been described as a nonmotile and nonflagellated organism. The previous statements that B. pertussis was a nonmotile organism were consistent with a stop codon located in the flagellar biosynthesis gene, flhA, discovered when the B. pertussis Tohama I genome was sequenced and analyzed by Parkhill et al. in 2003 (J. Parkhill, M. Sebaihia, A. Preston, L. D. Murphy, et al., Nat Genet, 35:32– 40, 2003, https://doi.org/10 .1038/ng1227). The stop codon has subsequently been found in all annotated genomes. Parkhill et al. also showed, however, that B. pertussis contains all genetic material required for flagellar synthesis and function. We and others have determined by various transcriptomic analyses that these flagellar genes are differentially regulated under a variety of B. pertussis growth conditions. In light of these data, we tested for B. pertussis motility and found that both laboratory-adapted strains and clinical isolates can be motile. Upon isolation of motile B. pertussis, we discovered flagellum-like structures on the surface of the bacteria. B. pertussis motility appears to occur primarily in the Bvg(2) phase, consistent with regulation present in B. bronchiseptica. Motility can also be induced by the presence of fetal bovine serum. These observations demonstrate that B. pertussis can express flagellum-like structures, and although it remains to be determined if B. pertussis expresses flagella during infection or if motility and/or flagella play roles during the cycle of infection and transmission, it is clear that these data warrant further investigation.
format	Articulo Articulo
author	Hoffman, Casandra L. Gonyar, Laura A. Zacca, Federico Sisti, Federico Fernández, Julieta Wong, Ting Damron, F. Heath Hewlett, Erik L.
author_facet	Hoffman, Casandra L. Gonyar, Laura A. Zacca, Federico Sisti, Federico Fernández, Julieta Wong, Ting Damron, F. Heath Hewlett, Erik L.
author_sort	Hoffman, Casandra L.
title	<i>Bordetella pertussis</i> Can Be Motile and Express Flagellum-Like Structures
title_short	<i>Bordetella pertussis</i> Can Be Motile and Express Flagellum-Like Structures
title_full	<i>Bordetella pertussis</i> Can Be Motile and Express Flagellum-Like Structures
title_fullStr	<i>Bordetella pertussis</i> Can Be Motile and Express Flagellum-Like Structures
title_full_unstemmed	<i>Bordetella pertussis</i> Can Be Motile and Express Flagellum-Like Structures
title_sort	<i>bordetella pertussis</i> can be motile and express flagellum-like structures
publishDate	2019
url	http://sedici.unlp.edu.ar/handle/10915/107762 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6520453&blobtype=pdf
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<i>Bordetella pertussis</i> Can Be Motile and Express Flagellum-Like Structures

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